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发帖数: 366 | 1 I cloned 400-500 bp miniarms cloned into ptdTomato-C1, and then linearized
it with EcoRI, so that the backbone was flanked by miniarms on both ends. I
electroporated the linearized plasmid into the induced Red recombinases in
SW102 carrying my BAC. All the clones turns to be empty. I succeed in the
other two using pKD46 system but failed in this one. And now I also fail
using SW102 system. What's the most possible reason? Thanks in advance. |
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