w******e
发帖数: 1187 | 1 covalent linked by EDC/NHS。想用一种universal的方法（非ELISA）测
beads上link的protein amount。因为beads本身会有interference，想要一种
assay方法，能根据protein amount给出signal，but the signal can't be
together w/ protein, so that the beads can be removed b4 measurement.
any thoughts? Thanks in advance! |
s******y
发帖数: 28562 | 2 我们一向都是采用倒推法。
就是看溶液里的蛋白减少了多少。
【在 w******e 的大作中提到】
: covalent linked by EDC/NHS。想用一种universal的方法（非ELISA）测
: beads上link的protein amount。因为beads本身会有interference，想要一种
: assay方法，能根据protein amount给出signal，but the signal can't be
: together w/ protein, so that the beads can be removed b4 measurement.
: any thoughts? Thanks in advance!
|
w******e
发帖数: 1187 | 3 thx for the input. I did that too. but when I tried to verify it
by directly measuring the proteins on beads, the result didn't add up...
【在 s******y 的大作中提到】
: 我们一向都是采用倒推法。
: 就是看溶液里的蛋白减少了多少。
|
