w******e 发帖数: 1187 | 1 covalent linked by EDC/NHS。想用一种universal的方法(非ELISA)测
beads上link的protein amount。因为beads本身会有interference,想要一种
assay方法,能根据protein amount给出signal,but the signal can't be
together w/ protein, so that the beads can be removed b4 measurement.
any thoughts? Thanks in advance! |
s******y 发帖数: 28562 | 2 我们一向都是采用倒推法。
就是看溶液里的蛋白减少了多少。
【在 w******e 的大作中提到】![](/moin_static193/solenoid/img/up.png) : covalent linked by EDC/NHS。想用一种universal的方法(非ELISA)测 : beads上link的protein amount。因为beads本身会有interference,想要一种 : assay方法,能根据protein amount给出signal,but the signal can't be : together w/ protein, so that the beads can be removed b4 measurement. : any thoughts? Thanks in advance!
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w******e 发帖数: 1187 | 3 thx for the input. I did that too. but when I tried to verify it
by directly measuring the proteins on beads, the result didn't add up...
【在 s******y 的大作中提到】![](/moin_static193/solenoid/img/up.png) : 我们一向都是采用倒推法。 : 就是看溶液里的蛋白减少了多少。
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