m******i
发帖数: 73 | 1 proteinase K 好象不行。 我想切成氨基酸，不是多肽。
请各位建议以下。 谢谢。 |
I*****y
发帖数: 6402 | 2 use trypsin to digest your protein overnight, and the amount of trypsin
needs to be higher to digest your protein into individual amino acids. my $0
.02
【在 m******i 的大作中提到】
: proteinase K 好象不行。 我想切成氨基酸，不是多肽。
: 请各位建议以下。 谢谢。
|
m******i
发帖数: 73 | 3 Trypsin usually cuts proteins at K or R. If I use higher concentration, is
it going to digest other amino acids?
Thanks.
$0
【在 I*****y 的大作中提到】
: use trypsin to digest your protein overnight, and the amount of trypsin
: needs to be higher to digest your protein into individual amino acids. my $0
: .02
|
p*******r
发帖数: 4048 | 4 http://www.piercenet.com/Objects/View.cfm?type=Page&ID=266C1558-BC35-4E01-
993C-2354B8B5AF26
is
【在 m******i 的大作中提到】
: Trypsin usually cuts proteins at K or R. If I use higher concentration, is
: it going to digest other amino acids?
: Thanks.
:
: $0
|
s******y
发帖数: 28562 | 5 The most complete way is hydrolysis in basic buffer and heating.
【在 m******i 的大作中提到】
: proteinase K 好象不行。 我想切成氨基酸，不是多肽。
: 请各位建议以下。 谢谢。
|
s******y
发帖数: 28562 | |
h*****t
发帖数: 1226 | 7 Why use enzyme, using hydrolysis, chemical treatment is much faster.
【在 m******i 的大作中提到】
: proteinase K 好象不行。 我想切成氨基酸，不是多肽。
: 请各位建议以下。 谢谢。
|
m******i
发帖数: 73 | 8 I need to detect some modifications on lysine. But the compound is not very
stable, which can not survive the harsh condition used for acid hydrolysis.
So, I have to use mild condition like enzyme digestion.
【在 h*****t 的大作中提到】
: Why use enzyme, using hydrolysis, chemical treatment is much faster.
|
h*****t
发帖数: 1226 | 9 If the lysine is modified, maybe enzyme can not cut around lysine residues
Proteinase K, and Pronase are pretty effiecnt enzyme to non-specifically
cut proteins
very
.
【在 m******i 的大作中提到】
: I need to detect some modifications on lysine. But the compound is not very
: stable, which can not survive the harsh condition used for acid hydrolysis.
: So, I have to use mild condition like enzyme digestion.
|
p******i
发帖数: 1092 | |
o*****e
发帖数: 9 | 11 proteasome + Tripeptidyl Peptidase II
【在 m******i 的大作中提到】
: proteinase K 好象不行。 我想切成氨基酸，不是多肽。
: 请各位建议以下。 谢谢。
|
K******S
发帖数: 10109 | 12 then why need to cut protein into amino acids? plenty other proteolytic
enzymes, you can even use trypsin, it will just cut the next K/R
very
.
【在 m******i 的大作中提到】
: I need to detect some modifications on lysine. But the compound is not very
: stable, which can not survive the harsh condition used for acid hydrolysis.
: So, I have to use mild condition like enzyme digestion.
|
