i***e
发帖数: 13 | 1 Hi- All,
I am having trouble with the simple tRNA aminoacylation reaction where E.
coli crude tRNA, E. coli S100 and 3H-Glu were added together with the AA
buffer and ATP. No charged tRNA could be detected. S100 and crude tRNA mix
have been tested to be active before in a similar experiment. The positive
control using Glu tRNA and active Glu -tRNA synthetase also didn't work. Is
anybody familiar with this reaction and suggest some possible reasons?
Thanks a lot! |
|
