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Biology版 - Summary for that Free Electron Laser
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1 (共1页)
o2
发帖数: 2764
1
I really get a free day today :) Get original post more than 100 follow up.
I am not try to encourage and discourage anything, just based on technique
itself.
It is a helpful tool for sure, but not gonna turn structure biology field
into a service center. Main reason is that we still need crystals, I think
anyone will X-ray background will agree on this.
So it will help on those hard to optimize crystals, but not for those easy
to optimize or hard to generate crystals protein.
As to crystals siz
J********n
发帖数: 534
2
I have a few questions 楼主:
1. What is the required crystal size? 10um? 1um? 100nm? 10nm?
2. How to solve the phase problem? Is the laser synchronized to the extent
that you know the phase information already?
3. How many micro crystals are needed for a complete dataset assuming only
one image is taken for each micro crystal?
4. How do you manually handle the crystals?

【在 o2 的大作中提到】
: I really get a free day today :) Get original post more than 100 follow up.
: I am not try to encourage and discourage anything, just based on technique
: itself.
: It is a helpful tool for sure, but not gonna turn structure biology field
: into a service center. Main reason is that we still need crystals, I think
: anyone will X-ray background will agree on this.
: So it will help on those hard to optimize crystals, but not for those easy
: to optimize or hard to generate crystals protein.
: As to crystals siz

1 (共1页)
进入Biology版参与讨论
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请教Nocodazole treat 的 NIH3T3 细胞该是啥样的呀?
egg donation对身体影响大吗?关于RNAi Knockdown的efficiency
transfection and cell cycle synchronization请教细胞同步化的方法(help -cell synchronization )
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