h********0
发帖数: 944 | 1 比如一些荧光蛋白如GFP会淬灭，有没有办法解决这个问题。我需要OVERNIGHT记录GFP
的表达变化。 |
s******y
发帖数: 28562 | 2 use the automatic trigger in your microscope to control the fluorescent
shutter
Use short time for each exposure and long intervals.
For example, I used to use 100ms exposure and 10 minute interval for
overnight recording
GFP
【在 h********0 的大作中提到】
: 比如一些荧光蛋白如GFP会淬灭，有没有办法解决这个问题。我需要OVERNIGHT记录GFP
: 的表达变化。
|
s******a
发帖数: 44 | 3 First, you need film a gfp fusion protein that express constantly (gfp alone
should work here) to test your system and setting for photo bleaching.
GFP
【在 h********0 的大作中提到】
: 比如一些荧光蛋白如GFP会淬灭，有没有办法解决这个问题。我需要OVERNIGHT记录GFP
: 的表达变化。
|
h********0
发帖数: 944 | 4 How can I make it? Do I need advanced microscope which can trig shutter
automatically?
【在 s******y 的大作中提到】
: use the automatic trigger in your microscope to control the fluorescent
: shutter
: Use short time for each exposure and long intervals.
: For example, I used to use 100ms exposure and 10 minute interval for
: overnight recording
:
: GFP
|
s*****j
发帖数: 6435 | 5 find the "time lapse" function in the control program. it should be self
explained.
【在 h********0 的大作中提到】
: How can I make it? Do I need advanced microscope which can trig shutter
: automatically?
|
s******y
发帖数: 28562 | 6 Yes, you should use a sysytem that has automatic trigger that is controled
by the image aquisition software. If you only have those manual switch, you
cannot take long time video of it
【在 h********0 的大作中提到】
: How can I make it? Do I need advanced microscope which can trig shutter
: automatically?
|
s******e
发帖数: 163 | 7 Or even longer interval, like 2 hours for florescence and 10 min for bright
field. |
