L**********r 发帖数: 143 | 1 Can someone comment on qPCR systems from ABI, BioRad, Stratagene, Eppendorf,
Roche, and Cepheid;
regarding cost, analysis software, reliability, customer support, etc. | c*********r 发帖数: 1312 | 2 同问,最近Illumina也推出了一款qPCR仪,不知道如何? | R****n 发帖数: 708 | 3 我用过这五种
CFX96 system,
Opticon2 system (Bio-rad),
7300 fast system,
Step one plus system (ABI)
Lightcycler II 480 (Roche).
我觉得如果不是做HRM,其实都差不多。不过我们组里都觉得Biorad CFX96比较user
friendly,但是出来的最晚。roche是用idaho的技术,资格比较老。ABI硬件很好,软件
很不好用。Rotorgene似乎温度控制的最好,不过没用的。能做HRM大概都是四五万块钱
.刚买没多久,还不知道售后怎么样。
http://hrm.gene-quantification.info/
Eppendorf,
【在 L**********r 的大作中提到】![](/moin_static193/solenoid/img/up.png) : Can someone comment on qPCR systems from ABI, BioRad, Stratagene, Eppendorf, : Roche, and Cepheid; : regarding cost, analysis software, reliability, customer support, etc.
| R****n 发帖数: 708 | 4 要是你自己用的话,还有一个事情比较重要。ABI和Roche都是用钨灯照射的,Gg过一两年要换,bio-rad是LED,基本上不用.bio-rad就像普通pcr仪可以开着overnight,第二天看结果。前两个一定要走前关掉,要不机器一晚上轰轰响。bio-rad不好的地方就是,升温降温比较慢。ABI和roche都可以 fast PCR,一个run也就是一个小时多点。
Eppendorf,
【在 L**********r 的大作中提到】![](/moin_static193/solenoid/img/up.png) : Can someone comment on qPCR systems from ABI, BioRad, Stratagene, Eppendorf, : Roche, and Cepheid; : regarding cost, analysis software, reliability, customer support, etc.
| L**********r 发帖数: 143 | 5 RinMan,
Thank you for the inputs.
The system will be mostly used for gene experssion profileling so HRM may be
a overkill; but still will look into it if the price differnce to the lower
end model is not big. | R****n 发帖数: 708 | 6 No problem. If you have some job information about DNA mutation/methylation analysis and Mass Spec analysis, that would be very helpful to me. Thank you!
be
lower
【在 L**********r 的大作中提到】![](/moin_static193/solenoid/img/up.png) : RinMan, : Thank you for the inputs. : The system will be mostly used for gene experssion profileling so HRM may be : a overkill; but still will look into it if the price differnce to the lower : end model is not big.
| w***a 发帖数: 4361 | 7 biorad CFX96一个run也就1小时多点吧。
这个机器还可以连上网络,设置一下,程序结束了直接email发结果。
两年要换,bio-rad是LED,基本上不用.bio-rad就像普通pcr仪可以开着overnight,第
二天看结果。前两个一定要走前关掉,要不机器一晚上轰轰响。bio-rad不好的地方就
是,升温降温比较慢。ABI和ro
【在 R****n 的大作中提到】![](/moin_static193/solenoid/img/up.png) : 要是你自己用的话,还有一个事情比较重要。ABI和Roche都是用钨灯照射的,Gg过一两年要换,bio-rad是LED,基本上不用.bio-rad就像普通pcr仪可以开着overnight,第二天看结果。前两个一定要走前关掉,要不机器一晚上轰轰响。bio-rad不好的地方就是,升温降温比较慢。ABI和roche都可以 fast PCR,一个run也就是一个小时多点。 : : Eppendorf,
| L**********r 发帖数: 143 | 8 One more question; is there any lab or core facility provide contract
service of RT-PCR?
methylation analysis and Mass Spec analysis, that would be very helpful to
me. Thank you!
【在 R****n 的大作中提到】![](/moin_static193/solenoid/img/up.png) : No problem. If you have some job information about DNA mutation/methylation analysis and Mass Spec analysis, that would be very helpful to me. Thank you! : : be : lower
| T**********t 发帖数: 1604 | 9 效果如何不知道,价钱是真便宜。list price才$13,900呢。
【在 c*********r 的大作中提到】![](/moin_static193/solenoid/img/up.png) : 同问,最近Illumina也推出了一款qPCR仪,不知道如何?
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