w******e 发帖数: 1187 | 1 一直用HRP-ABTS做ELISA,antibody signal强用的挺好。最近做aptamer
based ELISA,经常要develop1、2个小时,很是郁闷。请问有没有signal
amplification比较强劲的enzyme-substrate pair,产物是fluorescent
也行。//bow |
s******y 发帖数: 28562 | 2 Supersignal ELISA Femto
or Supersignal West FEMTO
both from Pierce Chemical (now under Fisher)
【在 w******e 的大作中提到】![](/moin_static193/solenoid/img/up.png) : 一直用HRP-ABTS做ELISA,antibody signal强用的挺好。最近做aptamer : based ELISA,经常要develop1、2个小时,很是郁闷。请问有没有signal : amplification比较强劲的enzyme-substrate pair,产物是fluorescent : 也行。//bow
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w******e 发帖数: 1187 | 3 赞!thx a lot!
【在 s******y 的大作中提到】![](/moin_static193/solenoid/img/up.png) : Supersignal ELISA Femto : or Supersignal West FEMTO : both from Pierce Chemical (now under Fisher)
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m*********7 发帖数: 5207 | 4 I recommend Immuno Pure TMB substrate kit from Pierce. |