S****2 发帖数: 164 | 1 1) non radioactive的EMSA kit发现pierce有卖,但文章很少,不知道能用不?
2) Chromatin IP,每次的sonication结果都不太一样,有时候200-1000 bp,有时候
1000 bp-genome size的smear
1% SDS根据millipore的EZ-ChIP自己配的
misonix 3000 with cuphorn adaptor
program: 10s On, 5s off, 30 cycles, 300ul sample volume, tube placed in
chilled water (if on wet ice,sds recipitates everytime within 1 minute)
谢先! |
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