About establishing stable transfected cell line - Biology版

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Biology版 - About establishing stable transfected cell line

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话题: cell话题: line话题: about话题: stable话题: gfp

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f**********s
发帖数: 1415

I plan to transfect GFP-fused target protein to my cell line and establish
stable cell line. I am wondering if there is neccessary to include negative
control (like salmon sperm DNA) or positive control (vector without encoding
GFP or target protein) during the transfection and selection process.
Thanks!

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● 慢病毒包装问题	● 请问哪里能买到transfection marker阿？
● CRISPR技术问题请教	● 电转求助（内有详细说明）
● MG 132 和cycloheximide 到底有啥用？哪位大神解释一下？	● macrophagy细胞transfection求助
● 韩春雨老师给的实验建议	● 快疯了～~Luciferase assay
● Arguing with Boss with co-transfection	● mouse stem cell siRNA transfection 求组
● 求助Hela transfection	● how to over-express a gene?
● transfection of suspension cells	● Co-IP 求助
● ESC line转染的问题	● 求一个retroviral transfection of suspension cell 的protocol

相关话题的讨论汇总
话题: cell话题: line话题: about话题: stable话题: gfp

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