When we say “copy number” of a plasmid, is it the average number of
plasmids per
cell or the maximum number of plasmids per cell?
It possible to lower the copy number of a certain plasmid by using less DNA
during
transformation?
Thanks!
X***n 发帖数: 366
2
1. Average number. There is little difference between average and maximum.
2. No. It depends on the replication origin of the plasmid.