N***P
发帖数: 153 | 1 我在调节人工脑脊液PH值的时候，每次加盐酸把PH降到7.0，不到1小时，PH回复到7.6-
7.7。连续几小时都这样。大家是怎么解决这个问题的？ | s*****c
发帖数: 103 | 2 ACSF is bicarbonate buffer (HCO3-/CO2), just bubble by 5%CO2/95%O2 for
plenty of time and test the pH, it will be around 7.3-7.4. So you will
need to keep bubbling the solution while using throughout experiments.
If you add HCl, the HCO3 will just become CO2 and released into air.
6-
【在 N***P 的大作中提到】
: 我在调节人工脑脊液PH值的时候，每次加盐酸把PH降到7.0，不到1小时，PH回复到7.6-
: 7.7。连续几小时都这样。大家是怎么解决这个问题的？
| N***P
发帖数: 153 | 3 谢谢，等会就去试试。
【在 s*****c 的大作中提到】
: ACSF is bicarbonate buffer (HCO3-/CO2), just bubble by 5%CO2/95%O2 for
: plenty of time and test the pH, it will be around 7.3-7.4. So you will
: need to keep bubbling the solution while using throughout experiments.
: If you add HCl, the HCO3 will just become CO2 and released into air.
:
: 6-
|
|
