h***a
发帖数: 145 | 1 what's the advantage of studying the role of a protein by transfecting
cells with this protein over studying naturally expressed proteins in
certain cells?
Thanks |
w******a
发帖数: 1527 | 2 Dumb answer: you can label the protein, or you can see the exogenous
expression versus the endogenous one? |
h*******g
发帖数: 711 | 3 To me, the exogenous-expressing system is good for mutagenesis study.... |
s**f
发帖数: 365 | 4 endogeneous的量太小或者只在特定条件下才表达
你overexpress的蛋白基本都是mutant或者磷酸化之类处理了的，用来研究表型或者蛋
白-蛋白相互作用。
【在 h***a 的大作中提到】
: what's the advantage of studying the role of a protein by transfecting
: cells with this protein over studying naturally expressed proteins in
: certain cells?
: Thanks
|
