l*****m 发帖数: 122 | 1 最近需要在老鼠里杀掉DC看看 但是又不能用DTR来做 想看看有没有直接用antibody
deplete DC的应用 我查到的基本都是in vitro条件的 想问问大家有没有谁做过in
vivo的呀?或者有推荐文献也行 谢谢啦! |
s****9 发帖数: 932 | 2 It is unlikely to use antibody to deplete DC in vivo.
【在 l*****m 的大作中提到】![](/moin_static193/solenoid/img/up.png) : 最近需要在老鼠里杀掉DC看看 但是又不能用DTR来做 想看看有没有直接用antibody : deplete DC的应用 我查到的基本都是in vitro条件的 想问问大家有没有谁做过in : vivo的呀?或者有推荐文献也行 谢谢啦!
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l*****m 发帖数: 122 | |
l*****m 发帖数: 122 | 4 对了 能具体说说原因么?
【在 s****9 的大作中提到】![](/moin_static193/solenoid/img/up.png) : It is unlikely to use antibody to deplete DC in vivo.
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s****9 发帖数: 932 | 5 I do not know. I assume that DC is very good at internalizing antigens and
bounded Ig can be easily internalized before successful ADCC occurs.
Similarly, I never see any reference depleting macrophages in vivo using mAb.
In addition, if CD11c-DTR cannot be used and macrophages play little role in
your system, you might consider clodronate liposome. High dose of CLL
depletes macrophages and most dendritic cells.
【在 l*****m 的大作中提到】![](/moin_static193/solenoid/img/up.png) : 对了 能具体说说原因么?
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s****9 发帖数: 932 | 6 Another alternative to consider is Flt3-ligand blockade. It remarkably
reduces DCs numbers, but you need to check more reference to see whether it
also influence some other myeloid compartments.
【在 l*****m 的大作中提到】![](/moin_static193/solenoid/img/up.png) : 最近需要在老鼠里杀掉DC看看 但是又不能用DTR来做 想看看有没有直接用antibody : deplete DC的应用 我查到的基本都是in vitro条件的 想问问大家有没有谁做过in : vivo的呀?或者有推荐文献也行 谢谢啦!
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s*****y 发帖数: 55 | 7 刚好看过这篇很有意思的文章:Effective collaboration between marginal
metallophilic macrophages and CD8+ dendritic cells in the generation of
cytotoxic T cells
用的就是楼上说的clodronate liposome。我用这东西除过macrophage,效果不错 |
p****l 发帖数: 291 | 8 in vivo不都是用
Steffen Jung或者Guenter hammerling的老鼠吗
你不能用DTR为啥?
【在 l*****m 的大作中提到】![](/moin_static193/solenoid/img/up.png) : 最近需要在老鼠里杀掉DC看看 但是又不能用DTR来做 想看看有没有直接用antibody : deplete DC的应用 我查到的基本都是in vitro条件的 想问问大家有没有谁做过in : vivo的呀?或者有推荐文献也行 谢谢啦!
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p****l 发帖数: 291 | 9 Flt3L不能deplete掉DCs,当然可以显著减少DCs数量
it
【在 s****9 的大作中提到】![](/moin_static193/solenoid/img/up.png) : Another alternative to consider is Flt3-ligand blockade. It remarkably : reduces DCs numbers, but you need to check more reference to see whether it : also influence some other myeloid compartments.
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