m******5
发帖数: 1383 | 1 A Bacterium That Can Grow by Using Arsenic Instead of Phosphorus
1. Felisa Wolfe-Simon1,2,*,
2. Jodi Switzer Blum2,
3. Thomas R. Kulp2,
4. Gwyneth W. Gordon3,
5. Shelley E. Hoeft2,
6. Jennifer Pett-Ridge4,
7. John F. Stolz5,
8. Samuel M. Webb6,
9. Peter K. Weber4,
10. Paul C. W. Davies1,7,
11. Ariel D. Anbar1,3,8 and
12. Ronald S. Oremland2
+ Author Affiliations
1.
1NASA Astrobiology Institute, USA.
2.
2U.S. Geological Survey, Menlo Park, CA, USA.
3.
3School of Earth and Space Exploration, Arizona State University,
Tempe, AZ, USA.
4.
4Lawrence Livermore National Laboratory, Livermore, CA, USA.
5.
5Department of Biological Sciences, Duquesne University, Pittsburgh,
PA, USA.
6.
6Stanford Synchrotron Radiation Lightsource, Menlo Park, CA, USA.
7.
7BEYOND: Center for Fundamental Concepts in Science, Arizona State
University, Tempe, AZ, USA.
8.
8Department of Chemistry and Biochemistry, Arizona State University,
Tempe, AZ, USA.
1. *To whom correspondence should be addressed. E-mail: felisawolfesimon@
gmail.com
Abstract
Life is mostly composed of the elements carbon, hydrogen, nitrogen, oxygen,
sulfur, and phosphorus. Although these six elements make up nucleic acids,
proteins, and lipids and thus the bulk of living matter, it is theoretically
possible that some other elements in the periodic table could serve the
same functions. Here, we describe a bacterium, strain GFAJ-1 of the
Halomonadaceae, isolated from Mono Lake, California, which substitutes
arsenic for phosphorus to sustain its growth. Our data show evidence for
arsenate in macromolecules that normally contain phosphate, most notably
nucleic acids and proteins. Exchange of one of the major bioelements may
have profound evolutionary and geochemical significance. | a*****g
发帖数: 543 | 2 I I remember the book. Some life in the North Pole right?
【在 m******5 的大作中提到】
: A Bacterium That Can Grow by Using Arsenic Instead of Phosphorus
: 1. Felisa Wolfe-Simon1,2,*,
: 2. Jodi Switzer Blum2,
: 3. Thomas R. Kulp2,
: 4. Gwyneth W. Gordon3,
: 5. Shelley E. Hoeft2,
: 6. Jennifer Pett-Ridge4,
: 7. John F. Stolz5,
: 8. Samuel M. Webb6,
: 9. Peter K. Weber4,
| S******G
发帖数: 965 | 3 “Our data show evidence for arsenate in macromolecules that normally
contain phosphate”
现在看不了全文，有人能说说是什么data吗？有analytical 的化学data吗？
structural biology 自然不必问了。
谢谢
【在 m******5 的大作中提到】
: A Bacterium That Can Grow by Using Arsenic Instead of Phosphorus
: 1. Felisa Wolfe-Simon1,2,*,
: 2. Jodi Switzer Blum2,
: 3. Thomas R. Kulp2,
: 4. Gwyneth W. Gordon3,
: 5. Shelley E. Hoeft2,
: 6. Jennifer Pett-Ridge4,
: 7. John F. Stolz5,
: 8. Samuel M. Webb6,
: 9. Peter K. Weber4,
| s******s
发帖数: 13035 | 4 基本上都是质谱啥的。多次传代只剩trace amount的磷。
【在 S******G 的大作中提到】
: “Our data show evidence for arsenate in macromolecules that normally
: contain phosphate”
: 现在看不了全文，有人能说说是什么data吗？有analytical 的化学data吗？
: structural biology 自然不必问了。
: 谢谢
| s******s
发帖数: 13035 | 5 复制以下我前面的帖子
原来觉得不太可思忆，不过看了一下，理解上大概砷和磷差别可能大一点，
但是砷酸和磷酸基团差别比较小，大的区别是砷酸很不稳定，但是这个细菌
在无磷环境下生长的时候会生许多大液泡，里面装聚-β-羟丁酸，这个玩意
能稳定砷酸。所以感觉上就是这个砷的替代物在聚-β-羟丁酸的环境下可能
就更加象磷酸基团了，所以这个细菌可能不需要每个相关分子都要做太大的
改变
【在 S******G 的大作中提到】
: “Our data show evidence for arsenate in macromolecules that normally
: contain phosphate”
: 现在看不了全文，有人能说说是什么data吗？有analytical 的化学data吗？
: structural biology 自然不必问了。
: 谢谢
| S******G
发帖数: 965 | 6 我的一个朋友给了我文章里面的数据。
1. 砷出现在protein，DNA这些里面不能说明他们对machinery和biochemistry有贡献。
换言之，这个细菌也许只用了其他10%的endogenous的100%含磷的agent来进行生化活动
。更退一步来说，如果there had been no phosphorus to begin with, 这些细菌肯定
活不下去。
2. 说明砷和磷一样是砷酸盐的形势存在。很好很强大，还是不能说明问题。
我觉得他们放卫星放得太早了点。
1. When the researchers added radioactively labeled arsenate to the bacteria
’s culture,they were later able to discern its presence in the protein,
lipid, nucleic acid, and metabolite fractions of the cells, suggesting that
arsenic had been incorporated in molecules forming each fraction.
2. They also separated out the DNA from the bacteria and analyzed
its composition using a technique called highresolution secondary ion mass
spectrometry; the isolated DNA contained arsenic. Tests utilizing the
intense x-rays at a synchrotron facility offered additional support,
indicating that at least some of the arsenic in the bacteria was in the form
of arsenate | s******s
发帖数: 13035 | 7 磷含量远远比你说的小，DNA，ATP这些你说的生化活动
都是砷参与的，本身细菌还是继续快速生长的，只是比全
用磷的时候稍慢而已。倒是kinase这些参与信号传导的倒
是可能仍然用磷。
bacteria
that
【在 S******G 的大作中提到】
: 我的一个朋友给了我文章里面的数据。
: 1. 砷出现在protein，DNA这些里面不能说明他们对machinery和biochemistry有贡献。
: 换言之，这个细菌也许只用了其他10%的endogenous的100%含磷的agent来进行生化活动
: 。更退一步来说，如果there had been no phosphorus to begin with, 这些细菌肯定
: 活不下去。
: 2. 说明砷和磷一样是砷酸盐的形势存在。很好很强大，还是不能说明问题。
: 我觉得他们放卫星放得太早了点。
:
: 1. When the researchers added radioactively labeled arsenate to the bacteria
: ’s culture,they were later able to discern its presence in the protein,
|
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