s**********d
发帖数: 1694 | 1 我要富集CD4-CD8- 的thymocytes,现在有两个办法，一个是sorter另外一个是MACS的
magnetic beads.我认为sorter的激光照射还有非常急的stream可能对细胞有伤害，而
macs因为是手动操作可以减少对细胞的伤害。但我老板说，magnetic的beads对细胞也
有伤害。不知有没有人有这方面的经验，能谈一下吗，谢谢了。 | s****9
发帖数: 932 | 2 No perfect methods. It largely depends on your research purpose.
Cells carrying beads are bad for their trafficking in vivo. But magnetic beads enrichment is quick and relatively economic, as compared to sorting. For in vitro study like RNA purification or culture, beads purification is fine.
Sorting is expensive and labor-intensive (I suppose your lab do not have
your own sorter and do not sort cell frequently). But without beads binding
, cells are more healthy.
【在 s**********d 的大作中提到】
: 我要富集CD4-CD8- 的thymocytes,现在有两个办法，一个是sorter另外一个是MACS的
: magnetic beads.我认为sorter的激光照射还有非常急的stream可能对细胞有伤害，而
: macs因为是手动操作可以减少对细胞的伤害。但我老板说，magnetic的beads对细胞也
: 有伤害。不知有没有人有这方面的经验，能谈一下吗，谢谢了。
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