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Biology版 - 请教蛋白质折叠结构的测定
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相关话题的讨论汇总
话题: structure话题: folding话题: sequence话题: protein话题: 3d
进入Biology版参与讨论
1 (共1页)
R*******N
发帖数: 7494
1
蛋白质折叠后的3D结构是用什么方法测定的?是测分子的位置然后重新人工组合么?
d****n
发帖数: 127
2
3D结构?X-ray或者NMR吧,前者要晶体,后者不能太大

【在 R*******N 的大作中提到】
: 蛋白质折叠后的3D结构是用什么方法测定的?是测分子的位置然后重新人工组合么?
R*******N
发帖数: 7494
3
Can you give some details? X-ray may detect rough structure, but how could
it detect the proteomic sequence and compare with the sequence before
folding?
I largely guess it needs to be confirmed by locating molecules such as
carbon, phos, or nitr, and arrange these molecules in 3D space?

【在 d****n 的大作中提到】
: 3D结构?X-ray或者NMR吧,前者要晶体,后者不能太大
f**e
发帖数: 2160
4
你能不能找本蛋白质结构的书翻一下再来问问题
p****s
发帖数: 3153
5
"The structure before folding", in the field of protein folding we don't
really have this concept...Or it is largely unimportant. Once the protein
nascent chain comes out ribosome, the folding has been already started.
Sure there are large families of proteins which are nativly unfolded. But
if you want to seek in vivo structure information, we just look at native
structure (the structure with the lowest overall or local energy) If you
want to study the unfolded structure (non native), you can use denature
methods, such as heat, urea and pH. They will shed light on the protein
stability and dynamics, and/or functions. For structural determination, we
will know the primary sequence in prior, then we can try to fit the
structure in Xray and apply the constraints in NMR.

could

【在 R*******N 的大作中提到】
: Can you give some details? X-ray may detect rough structure, but how could
: it detect the proteomic sequence and compare with the sequence before
: folding?
: I largely guess it needs to be confirmed by locating molecules such as
: carbon, phos, or nitr, and arrange these molecules in 3D space?

m*****n
发帖数: 421
6
If you only want an approxmate prediction, you might try SWISS-MODEL, a
online homology modelling server.
1 (共1页)
进入Biology版参与讨论
相关主题
Is there any dye that stains ribosomes?成熟的红细胞有没有 ribosome 啊?
到底为什么要做结构?Re: a bioinformatics question
荧光团标记蛋白质问题现在大家都用什么软件模拟protein structure
最近一周吃饭读science 上的新闻笔记RecBCD敲除
[合集] polyribosome的延长时间有什么实验方法可以比较啊Re: Anybody know this about Translation Initiation
有没有人搞蛋白质折叠的?学结构生物学的请进
如果只盯着一个蛋白,该怎么研究才能发好文章?为什么这个序列不是一个基因?
Postdoctoral Position Available (Northwestern University)哪位推荐一个anti-His tag的抗体,IP用
相关话题的讨论汇总
话题: structure话题: folding话题: sequence话题: protein话题: 3d