R*******N
发帖数: 7494 | 1 蛋白质折叠后的3D结构是用什么方法测定的？是测分子的位置然后重新人工组合么？ | d****n
发帖数: 127 | 2 3D结构？X-ray或者NMR吧，前者要晶体，后者不能太大
【在 R*******N 的大作中提到】
: 蛋白质折叠后的3D结构是用什么方法测定的？是测分子的位置然后重新人工组合么？
| R*******N
发帖数: 7494 | 3 Can you give some details? X-ray may detect rough structure, but how could
it detect the proteomic sequence and compare with the sequence before
folding?
I largely guess it needs to be confirmed by locating molecules such as
carbon, phos, or nitr, and arrange these molecules in 3D space?
【在 d****n 的大作中提到】
: 3D结构？X-ray或者NMR吧，前者要晶体，后者不能太大
| f**e
发帖数: 2160 | | p****s
发帖数: 3153 | 5 "The structure before folding", in the field of protein folding we don't
really have this concept...Or it is largely unimportant. Once the protein
nascent chain comes out ribosome, the folding has been already started.
Sure there are large families of proteins which are nativly unfolded. But
if you want to seek in vivo structure information, we just look at native
structure (the structure with the lowest overall or local energy) If you
want to study the unfolded structure (non native), you can use denature
methods, such as heat, urea and pH. They will shed light on the protein
stability and dynamics, and/or functions. For structural determination, we
will know the primary sequence in prior, then we can try to fit the
structure in Xray and apply the constraints in NMR.
could
【在 R*******N 的大作中提到】
: Can you give some details? X-ray may detect rough structure, but how could
: it detect the proteomic sequence and compare with the sequence before
: folding?
: I largely guess it needs to be confirmed by locating molecules such as
: carbon, phos, or nitr, and arrange these molecules in 3D space?
| m*****n
发帖数: 421 | 6 If you only want an approxmate prediction, you might try SWISS-MODEL, a
online homology modelling server. |
|
