x*******i
发帖数: 32 | 1 Hi there,
We have a protein that is soluble at room temperature but precipitates after
placing in refrigerator overnight. This is a reversible process, e.g.,
soluble again with temperature increase to room temperature.
Appreciate any idea or any possible explanation for it. What experiment
could be done to verify any hypothesis?
Thanks | z*h
发帖数: 773 | 2 The simplest explanation is solubility vs temperature. | s******s
发帖数: 13035 | 3 啥buffer呀？
化学里面也就一个溶解自由能的大小
after
【在 x*******i 的大作中提到】
: Hi there,
: We have a protein that is soluble at room temperature but precipitates after
: placing in refrigerator overnight. This is a reversible process, e.g.,
: soluble again with temperature increase to room temperature.
: Appreciate any idea or any possible explanation for it. What experiment
: could be done to verify any hypothesis?
: Thanks
| p*****n
发帖数: 981 | 4 这种现象也算常见
你把蛋白稀释10倍，看看是不是就好多了
after
【在 x*******i 的大作中提到】
: Hi there,
: We have a protein that is soluble at room temperature but precipitates after
: placing in refrigerator overnight. This is a reversible process, e.g.,
: soluble again with temperature increase to room temperature.
: Appreciate any idea or any possible explanation for it. What experiment
: could be done to verify any hypothesis?
: Thanks
|
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