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发帖数: 663 | 1 I plan to measure the calcium influx via a channel on cell membrane by
flurescence.
I found that furo 2 and fluro 3 might be the calcium indicator dyes to use.
Can someone give me some suggestions about which one to use? Is there any
difference for these two dyes? | h*********s
发帖数: 34 | 2 furo2 is ratiometric, which can be used to measure the Ca(II) concentrations.
fluro 3 is intensity based, which can only be used to show the relative
changes of Ca(II).
Also you should check if your fluorescent microscope can be used to read the
two excitation wavelength simultaneously for furo2. |
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