m******5
发帖数: 1383 | 1 I am thinking that ,theoretically, if the male gender ES cell are injected
and subsequently make it to germ line contribution, the chimera pups tend to
be in favor of Male gender type.
But, I don't know if it is true, anyone here who is more experienced on this
topic can tell me whether I am right or not ? |
i*****n
发帖数: 53 | 2 If the ES cells contributed to germ line, they should be able to convert
the gender type of the chimera pups. In another words, good Chimeras should
be male. |
s********r
发帖数: 312 | 3 If ES cells are male, only male chimeras are useful, vice versa. |
m******5
发帖数: 1383 | 4 Which means the sex ratio of chimera pups should tend to be predominantly
male?
should
【在 i*****n 的大作中提到】
: If the ES cells contributed to germ line, they should be able to convert
: the gender type of the chimera pups. In another words, good Chimeras should
: be male.
|
y*********u
发帖数: 183 | 5 I am having the same problem: some people here said that high agouti combine
with distorted sex ratio is usually a guarantee of successful germ line
transmission
but I am waiting for it to really happen -_ |
m******5
发帖数: 1383 | 6 Which cell line are you using ? I saw some literature mentioning that G4 or
Sv129 derived line is much more robust and with predictable behavior than C57 derived lines.
combine
【在 y*********u 的大作中提到】
: I am having the same problem: some people here said that high agouti combine
: with distorted sex ratio is usually a guarantee of successful germ line
: transmission
: but I am waiting for it to really happen -_
|
s********r
发帖数: 312 | 7 Yes,129 is the best strain for gene targeting. |
m******5
发帖数: 1383 | 8 -_- it is not my original question
【在 s********r 的大作中提到】
: Yes,129 is the best strain for gene targeting.
|
m******5
发帖数: 1383 | 9 ^_^ do you have an answer of my original answer?
I am waiting for an answer in an paranoid mode
【在 s********r 的大作中提到】
: Yes,129 is the best strain for gene targeting.
|
