m**********g 发帖数: 24 | 1 对一个基因的某一段序列(5‘utr)感兴趣,如何知道有哪些转录因子在上面有结合位
点?请高人指点。谢谢! |
b**********8 发帖数: 349 | |
m**********g 发帖数: 24 | 3 谢谢指教。非常有帮助。实验验证是要做CHiP 对吗? |
n******7 发帖数: 12463 | 4 TFBS指的是DNA promoter region
5'UTR是mRNA的一段,这怎么结合?
【在 m**********g 的大作中提到】![](/moin_static193/solenoid/img/up.png) : 对一个基因的某一段序列(5‘utr)感兴趣,如何知道有哪些转录因子在上面有结合位 : 点?请高人指点。谢谢!
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j****x 发帖数: 1704 | 5 TFBS也有在转录起始为点下游的情况,未必一定在promoter区域
【在 n******7 的大作中提到】![](/moin_static193/solenoid/img/up.png) : TFBS指的是DNA promoter region : 5'UTR是mRNA的一段,这怎么结合?
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n******7 发帖数: 12463 | 6 en,这也有可能。不过我还是怀疑lz的5'UTR指的是mRNA序列
【在 j****x 的大作中提到】![](/moin_static193/solenoid/img/up.png) : TFBS也有在转录起始为点下游的情况,未必一定在promoter区域
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a****o 发帖数: 1786 | 7 Most transcription factors bind to upstream of transcription start sites (
TSS).
General transcription factors bind to core promoter and activators/repressor
bind to UAS/enhancers.
5' UTR refers to regions in mRNA, but do not encode protein.It is not riched
for TF binding sites.
【在 j****x 的大作中提到】![](/moin_static193/solenoid/img/up.png) : TFBS也有在转录起始为点下游的情况,未必一定在promoter区域
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m**********g 发帖数: 24 | 8 我说的那一段序列确实是在转录起始点上游。谢谢各位的指教。受益匪浅!
再请教:
1 如何确定(查找)基因的转录起始点(transcription start site)?
2 如何确定(查找)基因的promoter? |
j****x 发帖数: 1704 | 9 The 5' UTR may also contain regulatory elements and may be part of the
promoter, and more and more updated TFBS searching tools include it into
analysis to increase the sensitivity. That's my point. I do have no problem
with the textbook knowledge you mentioned.
repressor
riched
【在 a****o 的大作中提到】![](/moin_static193/solenoid/img/up.png) : Most transcription factors bind to upstream of transcription start sites ( : TSS). : General transcription factors bind to core promoter and activators/repressor : bind to UAS/enhancers. : 5' UTR refers to regions in mRNA, but do not encode protein.It is not riched : for TF binding sites.
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