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Biology版 - 已经做过whole mount IHH的样品能用来做冰冻切片么?
相关主题
Re: Anybody know about immunofluorescence
求助IHC或者IF fixation的方法
cell surface marker staining 问题求助
请教做过chip on chip的实验室一个问题
about primary antibody
有没有能够识别phosporylated peptide的antibody啊??
需要试多个抗体的同学可以进来看一下。
大家elisa用的antibody一般都是从什么公司买的啊
how about antibody from genescript?
which company has good HA-tag antibody for WB and IP?
相关话题的讨论汇总
话题: antibody话题: mount话题: whole话题: ihh话题: fixation
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m******5
发帖数: 1383
1
不知道有人这么试验过么?
手头急需样品探路,下一批新鲜样平还要一周才能拿到…………
l*h
发帖数: 4124
2
yes, you can. but uneven fixation in whole mount is common. if your antibody
is sensitive to fixation depth, the results can be bad though.

【在 m******5 的大作中提到】
: 不知道有人这么试验过么?
: 手头急需样品探路,下一批新鲜样平还要一周才能拿到…………

m******5
发帖数: 1383
3
Thank you!!!

antibody

【在 l*h 的大作中提到】
: yes, you can. but uneven fixation in whole mount is common. if your antibody
: is sensitive to fixation depth, the results can be bad though.

l*h
发帖数: 4124
4
also make sure the antibodies used in your whole mount and the new
antibodies in frozen sections won't bind to each other.

【在 m******5 的大作中提到】
: Thank you!!!
:
: antibody

1 (共1页)
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相关主题
which company has good HA-tag antibody for WB and IP?
Santa Cruz的抗体好用吗?
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Re: Question about immunofluorescence
[合集] How to increase washing stringency in IP?
ChIP的问题
question about the EM
问一下methanol/acetic acid的fixation
相关话题的讨论汇总
话题: antibody话题: mount话题: whole话题: ihh话题: fixation