L******e 发帖数: 679 | 1 The following experiments were finished:
1: gene expression is increased significantly in tumor compared with the
corresponding normal tissue (RT-PCR for mRNA)
2: Protein expression in tumor cell is significantly increased (western blot)
3: Cell migration assay showed the stimulation in the overexpressing cells.
4; Gene silencing indicated the inhibition of some 'stree', which is related
to the gene.
What else need to do? Is thre a routine rule for the approval of an oncogene?
Thanks. |
C*****h 发帖数: 926 | 2 1. in vitro transformation assays (eg. soft agar assay, focus formation
assay)
2. in vivo over-expression to examine if tumor formation can be induced
blot)
related
oncogene?
【在 L******e 的大作中提到】![](/moin_static193/solenoid/img/up.png) : The following experiments were finished: : 1: gene expression is increased significantly in tumor compared with the : corresponding normal tissue (RT-PCR for mRNA) : 2: Protein expression in tumor cell is significantly increased (western blot) : 3: Cell migration assay showed the stimulation in the overexpressing cells. : 4; Gene silencing indicated the inhibition of some 'stree', which is related : to the gene. : What else need to do? Is thre a routine rule for the approval of an oncogene? : Thanks.
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b**********8 发帖数: 349 | 3
very good ideas~
I Cannot agree with you any more.
【在 C*****h 的大作中提到】![](/moin_static193/solenoid/img/up.png) : 1. in vitro transformation assays (eg. soft agar assay, focus formation : assay) : 2. in vivo over-expression to examine if tumor formation can be induced : : blot) : related : oncogene?
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N******n 发帖数: 3003 | 4 name that gene: Oncogene1 |
f*******e 发帖数: 354 | 5 is it a transcription factor?
combined with other oncogenes or anti-s, p53, sv40, e1f....in colony assay
KO or transgenic mice, if you got positive data from colony and
transplantation
what happened if you knockdown the gene in tumour cell lines, can you get
derive a cell line? or any other similar type of cancer cells
blot)
related
oncogene?
【在 L******e 的大作中提到】![](/moin_static193/solenoid/img/up.png) : The following experiments were finished: : 1: gene expression is increased significantly in tumor compared with the : corresponding normal tissue (RT-PCR for mRNA) : 2: Protein expression in tumor cell is significantly increased (western blot) : 3: Cell migration assay showed the stimulation in the overexpressing cells. : 4; Gene silencing indicated the inhibition of some 'stree', which is related : to the gene. : What else need to do? Is thre a routine rule for the approval of an oncogene? : Thanks.
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