s**********r 发帖数: 13 | 1 cre 始终和loxp配不到同一只小鼠中,cre,loxp和minis两两都可以配到一只小鼠中. |
c**********4 发帖数: 70 | 2 I know someone is using it. It seems they have problem. Mainly because it
expresses in testis and ovary. |
a******p 发帖数: 414 | 3 一个可能是: 你的cre和loxp在同一条染色体上, 这种情况很难拿到 cre; loxp/loxp
. 可能得大规模的筛选, 规模取决于cre 和loxp的距离。 |
s**********r 发帖数: 13 | 4 谢谢指点,偶尔得到基因型正确的小鼠(1%),WB验证却蛋白表达又没有变化.
loxp
【在 a******p 的大作中提到】 : 一个可能是: 你的cre和loxp在同一条染色体上, 这种情况很难拿到 cre; loxp/loxp : . 可能得大规模的筛选, 规模取决于cre 和loxp的距离。
|
s**********r 发帖数: 13 | 5 I want to konckout a transcription fact in testis,the problem is that Cre is
leaking.
【在 c**********4 的大作中提到】 : I know someone is using it. It seems they have problem. Mainly because it : expresses in testis and ovary.
|