p*******l
发帖数: 24 | 1 哪位有经验的说说，
该种多少细胞 （我用U2OS）？
是不是很难把细胞在chamber里均匀铺开（面积小）？
多谢指教。 | l******i
发帖数: 17 | 2 Look in Invitrogen Lipo2000, there is recommended number for cells needed.
Roughly it equals to the area of 24-well plate. | g*********5
发帖数: 2533 | 3 transfected them in 6 wells and after 24 hour trypsinzed cells , count, seed
them in chambers. | p*******l
发帖数: 24 | 4 Thanks a lot, laotouzi and giantbird05!!! |
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