m*********n
发帖数: 158 | 1 endogenous protein pulldown,以前都是harvest cell lysis，然后antibody incubation overnight, next day add
beads for 1-2hours, and pulldown
听说把beads先和antibody cross link一下，可以提高pulldown efficiency;
所以准备把antibody-bead cross link,加到cell lysis,这样得话，大家一般incubate
在cell lysis里面多久呀？
谢谢 | A******y
发帖数: 2041 | 2 Invitrogen crosslinking kit i.e. the co-immunoprecipitation kit recommended
no more than 1 hour. Some abs will not work once cross-linked, the
efficiency is not a guarantee but the background is a lot less. | m*********n
发帖数: 158 | |
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