f******9 发帖数: 22 | 1 Has anyone done three color for three different protein colocalization?
which second antibody did you choose?
I am quite confused with the multi choices from company. There are so many
fluorescent dyes. I mainly use Alexer from invitrogen. Has anyone work with
confocal before, any experience can share?
Thanks in advance. |
n******w 发帖数: 70 | 2 alexa系列都不错
cy系列的也不错
现在这些二抗质量都还很好的 尽管选一个系列的用就是了 |
f******9 发帖数: 22 | 3 Thanks. Which specific spectra do you think work best? since it is 3 colors? |
Z****9 发帖数: 738 | |
n******w 发帖数: 70 | 5 ALEXA 488, 555, 645
CY2, 3, 5
colors?
【在 f******9 的大作中提到】![](/moin_static193/solenoid/img/up.png) : Thanks. Which specific spectra do you think work best? since it is 3 colors?
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z****u 发帖数: 1007 | 6 4个颜色。
GFP, DAPI, Alex 568, Alex 647 as yellow.
many
with
【在 f******9 的大作中提到】![](/moin_static193/solenoid/img/up.png) : Has anyone done three color for three different protein colocalization? : which second antibody did you choose? : I am quite confused with the multi choices from company. There are so many : fluorescent dyes. I mainly use Alexer from invitrogen. Has anyone work with : confocal before, any experience can share? : Thanks in advance.
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C*******e 发帖数: 4348 | 7 好漂亮!
【在 z****u 的大作中提到】![](/moin_static193/solenoid/img/up.png) : 4个颜色。 : GFP, DAPI, Alex 568, Alex 647 as yellow. : : many : with
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c**n 发帖数: 73 | 8 Alex fluor
Dylight
Qdot
many
with
【在 f******9 的大作中提到】![](/moin_static193/solenoid/img/up.png) : Has anyone done three color for three different protein colocalization? : which second antibody did you choose? : I am quite confused with the multi choices from company. There are so many : fluorescent dyes. I mainly use Alexer from invitrogen. Has anyone work with : confocal before, any experience can share? : Thanks in advance.
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