f******9
发帖数: 22 | 1 Has anyone done three color for three different protein colocalization?
which second antibody did you choose?
I am quite confused with the multi choices from company. There are so many
fluorescent dyes. I mainly use Alexer from invitrogen. Has anyone work with
confocal before, any experience can share?
Thanks in advance. |
n******w
发帖数: 70 | 2 alexa系列都不错
cy系列的也不错
现在这些二抗质量都还很好的 尽管选一个系列的用就是了 |
f******9
发帖数: 22 | 3 Thanks. Which specific spectra do you think work best? since it is 3 colors? |
Z****9
发帖数: 738 | |
n******w
发帖数: 70 | 5 ALEXA 488, 555, 645
CY2, 3, 5
colors?
【在 f******9 的大作中提到】
: Thanks. Which specific spectra do you think work best? since it is 3 colors?
|
z****u
发帖数: 1007 | 6 4个颜色。
GFP, DAPI, Alex 568, Alex 647 as yellow.
many
with
【在 f******9 的大作中提到】
: Has anyone done three color for three different protein colocalization?
: which second antibody did you choose?
: I am quite confused with the multi choices from company. There are so many
: fluorescent dyes. I mainly use Alexer from invitrogen. Has anyone work with
: confocal before, any experience can share?
: Thanks in advance.
|
C*******e
发帖数: 4348 | 7 好漂亮！
【在 z****u 的大作中提到】
: 4个颜色。
: GFP, DAPI, Alex 568, Alex 647 as yellow.
:
: many
: with
|
c**n
发帖数: 73 | 8 Alex fluor
Dylight
Qdot
many
with
【在 f******9 的大作中提到】
: Has anyone done three color for three different protein colocalization?
: which second antibody did you choose?
: I am quite confused with the multi choices from company. There are so many
: fluorescent dyes. I mainly use Alexer from invitrogen. Has anyone work with
: confocal before, any experience can share?
: Thanks in advance.
|
