j******1 发帖数: 1315 | 1 包Lentivirus,但是如果用来感染Stem cell血清会对Stem Cell 有影响吧?才想到,
各位大侠该怎么办? |
a*****n 发帖数: 2835 | 2 纯化virus啊
【在 j******1 的大作中提到】![](/moin_static193/solenoid/img/up.png) : 包Lentivirus,但是如果用来感染Stem cell血清会对Stem Cell 有影响吧?才想到, : 各位大侠该怎么办?
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m*****z 发帖数: 1451 | 3 concentrate virus and resuspend in PBS
【在 j******1 的大作中提到】![](/moin_static193/solenoid/img/up.png) : 包Lentivirus,但是如果用来感染Stem cell血清会对Stem Cell 有影响吧?才想到, : 各位大侠该怎么办?
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z*t 发帖数: 863 | 4 use the serum containing medium is OK, we do MSCV all the time. As long as
your virus containing medium don't exceed 1/3 of final volume, then it's OK
【在 j******1 的大作中提到】![](/moin_static193/solenoid/img/up.png) : 包Lentivirus,但是如果用来感染Stem cell血清会对Stem Cell 有影响吧?才想到, : 各位大侠该怎么办?
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m*****z 发帖数: 1451 | 5 depends on the stem cell you want to transduce
as
OK
【在 z*t 的大作中提到】![](/moin_static193/solenoid/img/up.png) : use the serum containing medium is OK, we do MSCV all the time. As long as : your virus containing medium don't exceed 1/3 of final volume, then it's OK
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s**a 发帖数: 293 | 6 re
有些stem cell本身的培养基也是有血清的
【在 m*****z 的大作中提到】![](/moin_static193/solenoid/img/up.png) : depends on the stem cell you want to transduce : : as : OK
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