f*********8
发帖数: 129 | 1 要做mutation,譬如leucine,是 mutate to Ala?
这个mutate to what aa有什么讲究？
有啥书或文章介绍这些的？推荐一下。
谢谢。 | s******y
发帖数: 28562 | 2 这个大体都是经验值。
没有什么好讲究的，自己看一看侧链的结构就知道了。
【在 f*********8 的大作中提到】
: 要做mutation,譬如leucine,是 mutate to Ala?
: 这个mutate to what aa有什么讲究？
: 有啥书或文章介绍这些的？推荐一下。
: 谢谢。
| T****O
发帖数: 407 | 3 Usually you want to replace an AA because it's critical in your hypothesis.
Therefore, any perturbation would generate significant consequences. And
you want that significance to be as large as it could be theoretically
allowed. Mutating Leu to Ala is of the general practice. "Ala Scan",
remember? You could swap on Ala in replace of anything. The only exception
is Ala itself, which you should replace with Gly.
Now, it's important to notice the word "general". In many cases, simply
replacing an AA with Ala would not generate any Meaningful data. For
example, if in your hypothesis a critical AA is Asp and the enzyme lost
activity when you mutate it into Ala. How much could we learn from that?
Not that much. We don't really know all consequences for Asp/Ala being at
that particular place. In addition to the popular wish that the catalytic
site was gone, It could be the mutant didn't fold properly, or, the mutant
lost rigidity (or flexibility). Simply put it this way, you couldn't and
shouldn't rely on mutation method too much. And when you use the method,
design you experiment to cover as many facades as you could. |
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