o**4
发帖数: 35028 | |
h****6
发帖数: 229 | 2 About $200 from Genscript. I think others are similar. |
o**4
发帖数: 35028 | 3 Thanks
【在 h****6 的大作中提到】
: About $200 from Genscript. I think others are similar.
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o**4
发帖数: 35028 | 4 How much to synthesize 5kb DNA?
【在 h****6 的大作中提到】
: About $200 from Genscript. I think others are similar.
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t*********r
发帖数: 31 | |
m*****n
发帖数: 760 | 6 I used to order ~200bp, which cost me more than $100.
how did you get $99 for a 500bp oligo?
it would be great if you could share the experience. thanks
【在 t*********r 的大作中提到】
: $99.00 from IDT
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c********b
发帖数: 363 | 7 I just synthesized one by myself. Mine is 408 bp. I ordered 8 primers (
contains short complementary sequence one after another). The first 4 (
forward, reverse, forward, reverse to cover the first half of the sequence)
primers and the last 4 primers were put into 2 PCRs for annealing and
filling. The PCR products were then put into a new PCR for annealing and
filling. Then used the first forward primer and the last reverse primer for
a regular PCR. Gel purified the product, cloning. One day's job.
So I guess $99 IDT is the price for primers?? : )
【在 m*****n 的大作中提到】
: I used to order ~200bp, which cost me more than $100.
: how did you get $99 for a 500bp oligo?
: it would be great if you could share the experience. thanks
|
o**4
发帖数: 35028 | 8 靠，一天的工钱是多少啊？
)
for
【在 c********b 的大作中提到】
: I just synthesized one by myself. Mine is 408 bp. I ordered 8 primers (
: contains short complementary sequence one after another). The first 4 (
: forward, reverse, forward, reverse to cover the first half of the sequence)
: primers and the last 4 primers were put into 2 PCRs for annealing and
: filling. The PCR products were then put into a new PCR for annealing and
: filling. Then used the first forward primer and the last reverse primer for
: a regular PCR. Gel purified the product, cloning. One day's job.
: So I guess $99 IDT is the price for primers?? : )
|
M*****n
发帖数: 16729 | 9 $150
【在 h****6 的大作中提到】
: About $200 from Genscript. I think others are similar.
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o**4
发帖数: 35028 | 10 which company?
【在 M*****n 的大作中提到】
: $150
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w******n
发帖数: 767 | 11 为什么我在IDT合一个1.2kb的要近800刀，而且最后这帮人还没合出来。
【在 t*********r 的大作中提到】
: $99.00 from IDT
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x**2
发帖数: 255 | 12 没弄出来还收钱么？
【在 w******n 的大作中提到】
: 为什么我在IDT合一个1.2kb的要近800刀，而且最后这帮人还没合出来。
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w******n
发帖数: 767 | 13 他们倒想要，说给一个缺一块的序列，只收成本500还是多少，记不清了，没理他们。
【在 x**2 的大作中提到】
: 没弄出来还收钱么？
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b******y
发帖数: 627 | 14 Same thing happened to me. I switched to genewiz for gene synthesis ever
since. It is much better. Also the owner seems to be a Chinese.
【在 w******n 的大作中提到】
: 为什么我在IDT合一个1.2kb的要近800刀，而且最后这帮人还没合出来。
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h****6
发帖数: 229 | 15 What is your success rate with Genwiz? I am thinking about switching company
for my gene synthesis. Thanks,
【在 b******y 的大作中提到】
: Same thing happened to me. I switched to genewiz for gene synthesis ever
: since. It is much better. Also the owner seems to be a Chinese.
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t*********r
发帖数: 31 | 16 Here is the link for IDTDNA. They just launched the service less than a
month ago. You need to sequence the fragment but it is pretty high success
rate.
http://www.idtdna.com/pages/products/genes/gblocks-gene-fragmen
Good luck. |
x*******t
发帖数: 3764 | 17 为啥扩不出来呢？
500bp合成会有突变吧，还得折腾
【在 o**4 的大作中提到】
: 从基因组中扩增不出来，就想试试合成了，谢谢
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