g***j
发帖数: 40861 | |
C**S
发帖数: 522 | 2 先做个mini prep,看看产率怎么样
【在 g***j 的大作中提到】
: 昨天接种的时候忘记加抗生素了，质粒提还是不提啊
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g***j
发帖数: 40861 | 3 本来要提的就不多
干脆算了，今天重接种算了
【在 C**S 的大作中提到】
: 先做个mini prep,看看产率怎么样
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m****M
发帖数: 360 | 4 I specially tested overnight e coli cultures harboring plasmid with and
without antibotics, and turned out that I even got more plasmids than those
with antibotics. Try to isolate them (one or two if you don't want to waste
your miniprep column), and tell us your results. |
g***j
发帖数: 40861 | 5 如果等会儿有时间就提一下，不过以前没提过这个质粒，所以产率没法儿比较
those
waste
【在 m****M 的大作中提到】
: I specially tested overnight e coli cultures harboring plasmid with and
: without antibotics, and turned out that I even got more plasmids than those
: with antibotics. Try to isolate them (one or two if you don't want to waste
: your miniprep column), and tell us your results.
|
s*********9
发帖数: 9 | 6 还是不要提了，重新接种，重新摇，别忘了加抗生素。 |
g*********5
发帖数: 2533 | 7 my labmate asked plasmid from me and put it directly in LB, and she tried 3
times, no cloudy and asked me why.... |
g***j
发帖数: 40861 | 8 不至于吧，总得有点儿啊
另外时间不多，就没提
3
【在 g*********5 的大作中提到】
: my labmate asked plasmid from me and put it directly in LB, and she tried 3
: times, no cloudy and asked me why....
|
s******s
发帖数: 13035 | 9 没戏
【在 g***j 的大作中提到】
: 昨天接种的时候忘记加抗生素了，质粒提还是不提啊
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t**m
发帖数: 158 | 10 lol~
3
【在 g*********5 的大作中提到】
: my labmate asked plasmid from me and put it directly in LB, and she tried 3
: times, no cloudy and asked me why....
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|
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M*****n
发帖数: 16729 | 11 start over.
it will save you time. |
s*****g
发帖数: 7857 | 12 不！
【在 g***j 的大作中提到】
: 昨天接种的时候忘记加抗生素了，质粒提还是不提啊
|
w******n
发帖数: 767 | 13 谁这么有才把他弄进来的？
3
【在 g*********5 的大作中提到】
: my labmate asked plasmid from me and put it directly in LB, and she tried 3
: times, no cloudy and asked me why....
|
w**********g
发帖数: 1985 | 14 Kao, you are biological WSN
【在 g***j 的大作中提到】
: 昨天接种的时候忘记加抗生素了，质粒提还是不提啊
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l*****2
发帖数: 238 | |
g***j
发帖数: 40861 | 16 哈哈，我才反应过来
【在 w******n 的大作中提到】
: 谁这么有才把他弄进来的？
:
: 3
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