r*********y
发帖数: 124 | 1 试过几个FK2 anti-ub的抗体，背景都挺高（用2%BSA blocking）, 如果洗太久，信号
又太弱，请问谁有什么建议。谢谢 |
o**d
发帖数: 3080 | 2 for total ubiquitin, I use anti-ub antibody from santa cruz (P4D1).
Would you describe more about your experiment? |
r*********y
发帖数: 124 | 3 I'd like to examine mono-and poly-ub of drosophila endogenous protein after
IP. The protein is FLAG tagged and pulled down by M2 FLAG agarose beads (
sigma).
【在 o**d 的大作中提到】
: for total ubiquitin, I use anti-ub antibody from santa cruz (P4D1).
: Would you describe more about your experiment?
|
s******y
发帖数: 28562 | 4 对于 endogenous Ubiquitin 我用过最好的是 Millipore (MAB1510)
【在 r*********y 的大作中提到】
: 试过几个FK2 anti-ub的抗体，背景都挺高（用2%BSA blocking）, 如果洗太久，信号
: 又太弱，请问谁有什么建议。谢谢
|
