b******n
发帖数: 4225 | 1 俺的一个细菌，很小，大概直径0.5um，长5-10um
想通过做一个poteinX-EGFP的融合construct定位(可能是在periplasmic space)
什么样的confocal可以有这种sensitivity和resolution
哪个facility可以提供这种服务呢
谢谢啦 | p*l
发帖数: 1359 | 2 Try structured illumination microscope first (100 nm resolution). This kind
of microscope is not everywhere but you should be able to find it somewhere.
If structured illumination doesn't work, you need to use super resolution
methods. Very few places have them. You probably need to change your
labeling method depending on what method you want to use. GFP is not the best choice in super resolution. The only method that had been successful with regular GFP is STED. Other methods are PALM, STORM. | b******n
发帖数: 4225 | 3 thanks a lot
I have to study your information for a while:)
If I have questions, I'll continue to bother you
kind
somewhere.
best choice in super resolution. The only method that had been successful
with regular GFP is STED. Other methods are PALM, STORM.
【在 p*l 的大作中提到】
: Try structured illumination microscope first (100 nm resolution). This kind
: of microscope is not everywhere but you should be able to find it somewhere.
: If structured illumination doesn't work, you need to use super resolution
: methods. Very few places have them. You probably need to change your
: labeling method depending on what method you want to use. GFP is not the best choice in super resolution. The only method that had been successful with regular GFP is STED. Other methods are PALM, STORM.
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