a*****s
发帖数: 131 | 1 Hi everyone, I want to give mouse tamoxifen and look at ovary tissue. What
is the dose range and how to dissolve tamoxifen - it seems to be really
difficult in my hand?
Thank you every one for input!!! |
D*a
发帖数: 6830 | |
F*****e
发帖数: 182 | 3 1. 100mg tamoxifen in 1ml 100% ethanol, vortex for 2-5mins.
2. Add sesame oil to 10ml, leave it on a shaker until fully dissolved.
Aliquot into 500ul/tube and store tubes in -20oC for 1-6 months (final
concentration: 1mg/100ul).
3. Oral administration: 250-350ul/25g body weight. |
a*****s
发帖数: 131 | 4 Hi everyone, I want to give mouse tamoxifen and look at ovary tissue. What
is the dose range and how to dissolve tamoxifen - it seems to be really
difficult in my hand?
Thank you every one for input!!! |
D*a
发帖数: 6830 | |
F*****e
发帖数: 182 | 6 1. 100mg tamoxifen in 1ml 100% ethanol, vortex for 2-5mins.
2. Add sesame oil to 10ml, leave it on a shaker until fully dissolved.
Aliquot into 500ul/tube and store tubes in -20oC for 1-6 months (final
concentration: 1mg/100ul).
3. Oral administration: 250-350ul/25g body weight. |
a*****s
发帖数: 131 | 7 Thanks!
But I tried - after 1 hr shaking in sesame oil, why is there no any sign
that tamoxifen can be dissolved? How long does it usually take?
Thanks again!!
【在 F*****e 的大作中提到】
: 1. 100mg tamoxifen in 1ml 100% ethanol, vortex for 2-5mins.
: 2. Add sesame oil to 10ml, leave it on a shaker until fully dissolved.
: Aliquot into 500ul/tube and store tubes in -20oC for 1-6 months (final
: concentration: 1mg/100ul).
: 3. Oral administration: 250-350ul/25g body weight.
|
F*****e
发帖数: 182 | 8 usually around 20-30min. I don't know why yours didn't dissolve.
Where did you get the tamoxifen powder? Our tamoxifen and sesame oil are
from Sigma and they work well.
【在 a*****s 的大作中提到】
: Thanks!
: But I tried - after 1 hr shaking in sesame oil, why is there no any sign
: that tamoxifen can be dissolved? How long does it usually take?
: Thanks again!!
|
D*a
发帖数: 6830 | |
a*****s
发帖数: 131 | 10 My tamoxifen is from Sigma (Cat# T9262); how about yours?
My sesame oil is also from Sigma...
Thanks a lot - please help! Very frustrated...
【在 F*****e 的大作中提到】
: usually around 20-30min. I don't know why yours didn't dissolve.
: Where did you get the tamoxifen powder? Our tamoxifen and sesame oil are
: from Sigma and they work well.
|
h*******o
发帖数: 4884 | |
F*****e
发帖数: 182 | 12 Our tamoxifen is T5648. I checked T9262 and found it is "Tamoxifen citrate
salt", maybe that's the problem...
【在 a*****s 的大作中提到】
: My tamoxifen is from Sigma (Cat# T9262); how about yours?
: My sesame oil is also from Sigma...
: Thanks a lot - please help! Very frustrated...
|
a*****s
发帖数: 131 | 13 Great - thank you.
I ordered t5648 today.
【在 F*****e 的大作中提到】
: Our tamoxifen is T5648. I checked T9262 and found it is "Tamoxifen citrate
: salt", maybe that's the problem...
|
