h********n 发帖数: 4079 | 1 谁给推荐一个firefly luciferase的抗体, 用来老鼠组织的immunofluorescent.
谢谢谢谢. | Y*****e 发帖数: 9 | 2 Mouse anti-luciferase monoclonal antibody from Sigma (Cat#: L2164-.2ML
), try 1:200 dilution for IHC or IF. Use 1:400 or higher dilution for
western
【在 h********n 的大作中提到】![](/moin_static193/solenoid/img/up.png) : 谁给推荐一个firefly luciferase的抗体, 用来老鼠组织的immunofluorescent. : 谢谢谢谢.
| h********n 发帖数: 4079 | 3 thank you so much.
BTW, when you use it on mouse tissue, how is the specificity?
【在 Y*****e 的大作中提到】![](/moin_static193/solenoid/img/up.png) : Mouse anti-luciferase monoclonal antibody from Sigma (Cat#: L2164-.2ML : ), try 1:200 dilution for IHC or IF. Use 1:400 or higher dilution for : western
| Y*****e 发帖数: 9 | 4 You'd better use Vector Laboratories' MOM (mouse antibody on mouse tissue)
IHC kit since its blocking is better. Overall the antibody's is quite
specific.
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【在 h********n 的大作中提到】![](/moin_static193/solenoid/img/up.png) : thank you so much. : BTW, when you use it on mouse tissue, how is the specificity?
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