n***w 发帖数: 2405 | 1 最近在试一些junctional proteins, 如ZO-1, VE-Cadherin等等。。。
试了几个zymed (现invitrogen的),和DSHB的ZO-1,但是都不work。Western and IHC
都不行。
版上有人做过吗?
有些经验trick啥的吗?可以推荐下好用的抗体吗?谢谢 |
s******y 发帖数: 28562 | 2 Sigma 的monoclonal anti-PAN cadherin (cat# C1821) 还不错。
对VE-cadherin 肯定可以。我前几个星期刚在内皮细胞里试过。
注意要买这个单抗的。他们的那个多抗的质量太不稳定了,可把我坑苦了!
前一阵子还上来诉苦的。
IHC
【在 n***w 的大作中提到】![](/moin_static193/solenoid/img/up.png) : 最近在试一些junctional proteins, 如ZO-1, VE-Cadherin等等。。。 : 试了几个zymed (现invitrogen的),和DSHB的ZO-1,但是都不work。Western and IHC : 都不行。 : 版上有人做过吗? : 有些经验trick啥的吗?可以推荐下好用的抗体吗?谢谢
|
p*****e 发帖数: 332 | 3 Zo1我们用invitrogen Mouse的,还不错,但是兔子的不work.
IHC
【在 n***w 的大作中提到】![](/moin_static193/solenoid/img/up.png) : 最近在试一些junctional proteins, 如ZO-1, VE-Cadherin等等。。。 : 试了几个zymed (现invitrogen的),和DSHB的ZO-1,但是都不work。Western and IHC : 都不行。 : 版上有人做过吗? : 有些经验trick啥的吗?可以推荐下好用的抗体吗?谢谢
|
n***w 发帖数: 2405 | 4 前面没说清楚,
我就是要染老鼠tissue,所以我尽量避免mouse mAb...
【在 p*****e 的大作中提到】![](/moin_static193/solenoid/img/up.png) : Zo1我们用invitrogen Mouse的,还不错,但是兔子的不work. : : IHC
|
n***w 发帖数: 2405 | 5 谢谢嗯,我是想染whole retina嗯,所以避免单抗。。
【在 s******y 的大作中提到】![](/moin_static193/solenoid/img/up.png) : Sigma 的monoclonal anti-PAN cadherin (cat# C1821) 还不错。 : 对VE-cadherin 肯定可以。我前几个星期刚在内皮细胞里试过。 : 注意要买这个单抗的。他们的那个多抗的质量太不稳定了,可把我坑苦了! : 前一阵子还上来诉苦的。 : : IHC
|
s******y 发帖数: 28562 | 6 oh....那就没办法了。。。
Sigma 也有Rabbit anti PAN cadherin,但是是个多抗,每一批都不一样!
倒是每次都能做WB 是没有问题的,染经典的E-cadherin 也每批都行,
就是染N, VE 不是每批都行
【在 n***w 的大作中提到】![](/moin_static193/solenoid/img/up.png) : 前面没说清楚, : 我就是要染老鼠tissue,所以我尽量避免mouse mAb...
|
p*****e 发帖数: 332 | 7 那就不知道了,不好意思,我也是初学者
【在 n***w 的大作中提到】![](/moin_static193/solenoid/img/up.png) : 前面没说清楚, : 我就是要染老鼠tissue,所以我尽量避免mouse mAb...
|
g***y 发帖数: 201 | 8 我们用很多mouse antibody 染老鼠tissue...
【在 n***w 的大作中提到】![](/moin_static193/solenoid/img/up.png) : 前面没说清楚, : 我就是要染老鼠tissue,所以我尽量避免mouse mAb...
|
|
n***w 发帖数: 2405 | 9 嗯,你们是怎么处理的?因为我要染血管,所以比较担心。。。。
用no primary做对照?还是用了什么特别的kit,比如说vector labs的m.o.m?
谢谢。
真希望有些GFP junctional protein mice。
【在 g***y 的大作中提到】![](/moin_static193/solenoid/img/up.png) : 我们用很多mouse antibody 染老鼠tissue...
|
g***y 发帖数: 201 | 10
we stain brain tissues.we perfuse mouse first
yes
【在 n***w 的大作中提到】![](/moin_static193/solenoid/img/up.png) : 嗯,你们是怎么处理的?因为我要染血管,所以比较担心。。。。 : 用no primary做对照?还是用了什么特别的kit,比如说vector labs的m.o.m? : 谢谢。 : 真希望有些GFP junctional protein mice。
|
l**********1 发帖数: 5204 | 11 RE: 德艺双馨的楼主
please go to
//www.creativebiomart.net/description_10243_28.htm
You Position: Home >> Antibody >> Polyclonal Antibody >> Rabbit PAb >> Anti-Cdh5 PAb
Anti-Cdh5 PAb
Cat. No.
CPB-133RM
>
Immunogen
Recombinant mouse CDH5 protein.
Antibody Type
Rabbit Polyclonal Antibody.
Ig Type
Rabbit IgG.
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Preparation
Produced in rabbits immunized with purified, human cell-derived, recombinant mouse CDH5 extracellular
domain (Met 1-Ala 592). Total IgG was purified by Protein A affinity chromatography.
Specificity
Mouse CDH5/VE-Cad/CD144.
Direct ELISA
This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse
CDH5. The detection limit for mouse CDH5 is 0.0195 ng/well.
Storage
This antibody can be stored at 2oC-8oC for one month without detectable loss of activity. Antibody
products are stable for twelve months from date of receipt when stored at -20oC to -70oC. Preservative-
Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to
cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
ps:
CC one mail from there:
Dear Sir/Madam,
Creative Biomart is delighted to announce the launch of 1,000 brand new
products in a large number of research areas including GPCR, Neuroscience,
Cancer, Cardiovascular, Immunology, Cell Biology, Chromatin and Nuclear
Signaling, Developmental Biology, Microbiology, Signal Transduction,Stem
Cells, and many more.
>200 new purified and recombinant proteins
>500 new monoclonal antibodies
>150 new polyclonal antibody
ELISA kits targeting over 150 new targets
All of our products have full technical datasheets, with usage
recommendations and online protocols.Search for and order your products
ONLINE and take advantage of our new enhanced searching and filtering to
quickly and easily find products that fit your requirements.
We offer bulk pricing on many of our products and will be delighted to help
you with any specific product requests. Email us at info@creative-biomart.
org or call us on 631-559-9269.
----------------------------------------------------------------------------
--------------------------------------------------------
Best regards,
Angie Lee, Ph.D.
Account Manager
45-16 Ramsey Road Shirley, NY 11967, USA
Tel: 1-631-559-9269
E-mail: info (at) creative-biomart.org
//www.creativebiomart.net/
【在 n***w 的大作中提到】![](/moin_static193/solenoid/img/up.png) : 谢谢嗯,我是想染whole retina嗯,所以避免单抗。。
|