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Biology版 - adenoviral cre or lentiviral cre for mouse primary cells?
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相关话题的讨论汇总
话题: cre话题: adenoviral话题: lentiviral话题: cells话题: mouse
进入Biology版参与讨论
1 (共1页)
t***i
发帖数: 114
1
哪种Cre少点细胞毒性?我主要做in vitro的细胞培养。谢谢了!
n********k
发帖数: 2818
2
it depends on the titre, infection efficiency, promoter and ur system etc..
generally speaking ad-cre can be more effective but with higher toxicity too
...I would go with lenti-cre if it is effective enough but for some stem
cells, it might not work well...

【在 t***i 的大作中提到】
: 哪种Cre少点细胞毒性?我主要做in vitro的细胞培养。谢谢了!
t***i
发帖数: 114
3
I see, got it. I'll try lenti-cre first. Thanks a lot!

too

【在 n********k 的大作中提到】
: it depends on the titre, infection efficiency, promoter and ur system etc..
: generally speaking ad-cre can be more effective but with higher toxicity too
: ...I would go with lenti-cre if it is effective enough but for some stem
: cells, it might not work well...

1 (共1页)
进入Biology版参与讨论
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做实验室 碰到伤口了lentiviral based Cell line 求助
用lentiviral vector对操作者的危害有多大啊?如何提高工具病毒感染组织的效率?请支招
如何transfect siRNA into MEFsmammalian cells transfection 后 integration 的效率大概是多少?
Has anyone made pGIPZ lentiviral KD construction before?lentiviral shRNA infection on primary T cells
Technique请教:human T lymphocytes - gene delivery and expressionlentiviral infected cell line 的安全
Adenovirus, Retrovirus, 和Lentivirus 的表达快慢?请推荐vector/cell line
同时表达两个shRNA的lentiviral vector请推荐Generation of adenovirus的公司(adenoviral construct provided)
Can retroviral vector be used for lentiviral packaging and infection?30kb的大质粒,应该如何转染?
相关话题的讨论汇总
话题: cre话题: adenoviral话题: lentiviral话题: cells话题: mouse