x********u 发帖数: 430 | 1 I have been running SDS-PAGE several times and cannot get clear band for low
molecular proteins. Any suggestions will be highly appreciated.
Attached is a gel picture I just took yesterday. (12% resolving gel and load
50 ug total E. coli cell lysate proteins). |
p*****p 发帖数: 19331 | 2 自己配的胶?买的?
可能是胶不好,也可能过热
可以降低电压,放冰盒里跑 |
x*****o 发帖数: 441 | 3 用tricine sds page好像对分子量小的蛋白效果比较好.
low
load
【在 x********u 的大作中提到】![](/moin_static193/solenoid/img/up.png) : I have been running SDS-PAGE several times and cannot get clear band for low : molecular proteins. Any suggestions will be highly appreciated. : Attached is a gel picture I just took yesterday. (12% resolving gel and load : 50 ug total E. coli cell lysate proteins).
|
c*z 发帖数: 157 | 4 12%会不会太浓了?我一直以为梯度胶选择4-12%是为了防丢东西的 下面根本分不出来
曾经在一个有钱的lab,跑小蛋白 用invitrgen bis tris 6% precasted gel, bis-
tris buffer
膜用PVDF, 不用NC的,怕丢
【在 x*****o 的大作中提到】![](/moin_static193/solenoid/img/up.png) : 用tricine sds page好像对分子量小的蛋白效果比较好. : : low : load
|
D*a 发帖数: 6830 | 5 我们也在摸条件,前几次用的16.5%的SDS-PAGE跑6KDa的蛋白。有条带但是比较粗,下
边的smear不知道是什么。
今天同时用了4-20%梯度的,梯度的明显跑的好,第一次看见marker的4kDa的带。以前
16.5%看不见这条带。
等结果出来再上来汇报汇报。 |