s******a 发帖数: 472 | |
y*****w 发帖数: 146 | 2 you just need to sort 5x10^8-10^9 cells to get your positive clone.
【在 s******a 的大作中提到】![](/moin_static193/solenoid/img/up.png) : 100万个细胞中有1个阳性细胞能分出来吗?
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s******a 发帖数: 472 | 3 相当于500到1000个阳性细胞
我想知道我能分出来多少呢?
【在 y*****w 的大作中提到】![](/moin_static193/solenoid/img/up.png) : you just need to sort 5x10^8-10^9 cells to get your positive clone.
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y*****w 发帖数: 146 | 4 sorry, I miscalculated, actually, you should be able to get your
positive
clones if you have around 1-5 in the total cells (means you just need to
sort 5x10^6 cells).
The only potential problem is how you differentiate the positive clones
from
the negative ones. If the sensitivity is good enough, you theoretically
just need one clone. Normally, 5-fold should be safe enough for you to
get
one unless your have a very crappy assay.
It all depends on your assay. I would say 10-fold less of the total
positive clones is a safe number you should be able to get. |
w***a 发帖数: 4361 | 5 得看抗体好不好,细胞总数如果有几十亿,那还是还机会的。
【在 s******a 的大作中提到】![](/moin_static193/solenoid/img/up.png) : 100万个细胞中有1个阳性细胞能分出来吗?
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y***j 发帖数: 11235 | 6 十几个每M我做过,是可以的,然后PCR检测,基本上能和流失结果对得上。
但是效率很低,保守估计只能得到1/5的细胞,当然也可能是我们的core太烂,很多人
骂。
【在 s******a 的大作中提到】![](/moin_static193/solenoid/img/up.png) : 100万个细胞中有1个阳性细胞能分出来吗?
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