q**********0
发帖数: 335 | 1 Recently, I transfected pmaxGFP plasmid to HepG2 cells by using
Lipofectamine2000 to track the positive-transfected cells with another test
plasmid. However, the FACS result showed no separation between GFP positive
or negative cells, only one cell population. I don't know whether the
transfection efficiency is too high that almost every cell was transfected?
or pmaxGFP is not a proper GFP plasmid to use in FACS? Your suggestions will
be appreciated. | a****d
发帖数: 1919 | 2 it should work for flow
make sure you have no-transfected control population
do several different transfection dose may also help to tell the difference | q**********0
发帖数: 335 | 3 Thanks.
difference
【在 a****d 的大作中提到】
: it should work for flow
: make sure you have no-transfected control population
: do several different transfection dose may also help to tell the difference
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