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Biology版 - 有哪个microRNA一表达细胞就死翘翘的
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话题: microrna话题: 死翘翘话题: 细胞话题: 筛选
进入Biology版参与讨论
1 (共1页)
o**i
发帖数: 1165
1
想用它来做附筛选的marker
大家有什么建议么
m******5
发帖数: 1383
2

Do you have to use microrna?

【在 o**i 的大作中提到】
: 想用它来做附筛选的marker
: 大家有什么建议么

b******y
发帖数: 627
3
does it exist?
b******y
发帖数: 627
4
how about high concentration of polyT?
w********r
发帖数: 1431
5
有一个siRNA是可以的,不记得是啥了。invitrogen的RNAi library用这个当positive
ctrl。如果是miRNA筛选的话或者可能也能用?
R***R
发帖数: 166
6
怎么导入呢?

【在 b******y 的大作中提到】
: how about high concentration of polyT?
b******y
发帖数: 627
7
Not my expertise. That was just a question out of my curiosity.
Nevertheless, can it be cloned into pcDNA3 and letivirus with tetracycline
controllable promoter for transfection or infection (depending on the cell
types in use)?
b******s
发帖数: 5365
8
Kif11,our lab used it before, it kills cells, it is used for transfection
control

positive

【在 w********r 的大作中提到】
: 有一个siRNA是可以的,不记得是啥了。invitrogen的RNAi library用这个当positive
: ctrl。如果是miRNA筛选的话或者可能也能用?

1 (共1页)
进入Biology版参与讨论
相关主题
Difficult cloning - 求助请推荐In Vitro Transcription / Translation kits
stable clones vs. stable poolNews: Mammalian microRNAs predominantly act to decrease target mRNA levels
有没有人用multisite gateway做克隆的?Real time PCR 内参照引物设置 ?
再问gateway cloningA difficult question
请教microRNA 表达Plant RNAs Found in Mammals The-Scientists Highlights
Help! clone large fragment into pLenti7.3/V5 TOPOmicroRNA相关问题询问
Re: Sequence我是张辰宇的学生,说说最近的事
关于microRNA的问题这算不算打脸?MicroRNAs are digested, not absorbed
相关话题的讨论汇总
话题: microrna话题: 死翘翘话题: 细胞话题: 筛选