a******a
发帖数: 283 | 1 Wonder anybody has experience with it and what's the perspective? I only
know the one made by Bio-rad. It seems like there is another company (don't
remember the name) also claims they have this technology.
Any insight of any sort? |
s******s
发帖数: 13035 | 2 你是说raindance么？和454, ion torrent都是一牛发明的
t
【在 a******a 的大作中提到】
: Wonder anybody has experience with it and what's the perspective? I only
: know the one made by Bio-rad. It seems like there is another company (don't
: remember the name) also claims they have this technology.
: Any insight of any sort?
|
c********4
发帖数: 111 | 3 Raindance has smaller droplets (more partitions), but the reading time is
way too long and their product seems not available yet.
Life Tech also has a new one (chamber mechanism as fluidigm , rather than
droplets), don't know about the performance.
Bio-Rad's ddPCR is from previous Quantalife, a lot cases and applications
accumulation with lower price. |
b******k
发帖数: 2321 | 4 quantalife 最近恰好看到这个
t
【在 a******a 的大作中提到】
: Wonder anybody has experience with it and what's the perspective? I only
: know the one made by Bio-rad. It seems like there is another company (don't
: remember the name) also claims they have this technology.
: Any insight of any sort?
|
h******y
发帖数: 351 | 5 Raindance和Bio-Rad的ddPCR的原理是不一样的。
Raindance出来的结果不直观，而且不是绝对定量。
ddPCR定量的原理利用的是泊松分布，在低浓度范围内定量挺准，缺点是定量范围比较
窄。
【在 c********4 的大作中提到】
: Raindance has smaller droplets (more partitions), but the reading time is
: way too long and their product seems not available yet.
: Life Tech also has a new one (chamber mechanism as fluidigm , rather than
: droplets), don't know about the performance.
: Bio-Rad's ddPCR is from previous Quantalife, a lot cases and applications
: accumulation with lower price.
|
