e**r 发帖数: 1144 | 1 好象是histone modification.
谁知道原始的出处? | A******y 发帖数: 2041 | 2 It should be a pretty recent paper. Lysine crotonic modification is not
known until late 2011. | f******9 发帖数: 39 | | s******s 发帖数: 13035 | 4 不会用google image search?
https://www.google.com/search?tbs=sbi:
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【在 e**r 的大作中提到】![](/moin_static193/solenoid/img/up.png) : 好象是histone modification. : 谁知道原始的出处?
| S******i 发帖数: 71 | 5 据某生化大牛说, 蛋白croto似乎只是一个垃圾, 蛋白修饰很多,但是是不是生物学修饰
,还是化学性修饰,这点是修饰的关键. 据说蛋白croto只是一些化学修饰,其报道的表观
学意义是个artificial,这也是蛋白croto发表之后跟风的文章很少,应该是不能重复的
原因吧.
【在 A******y 的大作中提到】![](/moin_static193/solenoid/img/up.png) : It should be a pretty recent paper. Lysine crotonic modification is not : known until late 2011.
| X*********4 发帖数: 50 | 6 楼下说的对, 图里面的crotonylted是Zhao yingming他们发现的. 但是我看着很solid
的样子, 不可能是造假的噢
【在 e**r 的大作中提到】![](/moin_static193/solenoid/img/up.png) : 好象是histone modification. : 谁知道原始的出处?
| A******y 发帖数: 2041 | 7 It really doesn't matter if it is chemical modification or biological
modification. Some scientist in the field believe a lot of acetylation
modification is through direct chemical transfer of Acetyl-CoA without the
help of HAT espeically in organelle enviroment favoring nucleophilic
addition.
As far as crotonyl-modification. My friesd published in a good journal
stating HDAC3 is the de-crotonylase...my lab results are exactly opposite.
HDAC1 and HDAC2 have more activities against cortonyl-lysine in my hand (hey
, I didn't review that paper, don't blame me). In addition, the Kcat is
very low compare to acetylation substrate, so I don't know if it is really
biologically relevent.
【在 S******i 的大作中提到】![](/moin_static193/solenoid/img/up.png) : 据某生化大牛说, 蛋白croto似乎只是一个垃圾, 蛋白修饰很多,但是是不是生物学修饰 : ,还是化学性修饰,这点是修饰的关键. 据说蛋白croto只是一些化学修饰,其报道的表观 : 学意义是个artificial,这也是蛋白croto发表之后跟风的文章很少,应该是不能重复的 : 原因吧.
| S******i 发帖数: 71 | 8 按照这个做法, 蛋白修饰是很多很多了, 就像蛋白解构, 每个蛋白都有解构, 也是重要
; 关键还是要有生物功能. 所以修饰的生命特征是后续研究重点, 如果following
biological significane没有文章来解析, 说明很多问题, 最大可能不太可靠的
hey
【在 A******y 的大作中提到】![](/moin_static193/solenoid/img/up.png) : It really doesn't matter if it is chemical modification or biological : modification. Some scientist in the field believe a lot of acetylation : modification is through direct chemical transfer of Acetyl-CoA without the : help of HAT espeically in organelle enviroment favoring nucleophilic : addition. : As far as crotonyl-modification. My friesd published in a good journal : stating HDAC3 is the de-crotonylase...my lab results are exactly opposite. : HDAC1 and HDAC2 have more activities against cortonyl-lysine in my hand (hey : , I didn't review that paper, don't blame me). In addition, the Kcat is : very low compare to acetylation substrate, so I don't know if it is really
| X*********4 发帖数: 50 | 9 估计是Zhao 实验室找到的蛋白酶? 其实你发现的和别人发现的有分歧,怎么办? 是实验
室系统的问题,
我觉得实验材料问题很大吧, 化学蛋白组实验室对蛋白样品的质量很重视,但是对生物
材料的质量和来源并不重视. 所以他们的发现能不能在其他生物系统中重复是关键, 重
复不出来的估计就是垃圾了, 至于能不能算造假,不知道.
hey
【在 A******y 的大作中提到】![](/moin_static193/solenoid/img/up.png) : It really doesn't matter if it is chemical modification or biological : modification. Some scientist in the field believe a lot of acetylation : modification is through direct chemical transfer of Acetyl-CoA without the : help of HAT espeically in organelle enviroment favoring nucleophilic : addition. : As far as crotonyl-modification. My friesd published in a good journal : stating HDAC3 is the de-crotonylase...my lab results are exactly opposite. : HDAC1 and HDAC2 have more activities against cortonyl-lysine in my hand (hey : , I didn't review that paper, don't blame me). In addition, the Kcat is : very low compare to acetylation substrate, so I don't know if it is really
| e**r 发帖数: 1144 | |
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