u*********1
发帖数: 2518 | 1 对一个novel gene有兴趣。
准备想用病人的lymphoblast来研究。因为最容易得到。
但问题是，去UCSC browser现实这个基因在lymphoblast里几乎不表达；但因为是神经
相关的基因所以肯定在CNS里表达
Questions:
1. 很stupid的问题，如果想知道一个基因的expression level，该查阅啥database？
我看的是UCSC里的 “Transcription Levels Assayed by RNA-seq on 9 Cell Lines
from ENCODE”
2. 如果lymphoblast里表达量很低，what shall I do next? | A******y
发帖数: 2041 | 2 How about GEO database? If it is a brain tissue, you can try Hek293 (I know
it is a kidney cell line), but for some unknown reasons kidney is very
similar to brain. |
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