g*********5
发帖数: 2533 | 1 换了几种medium，要么长的慢，要么集团。谢谢 | s*****g
发帖数: 7857 | | a*****n
发帖数: 2835 | 3 follow ATCC instruction
这细胞好像就这样
【在 g*********5 的大作中提到】
: 换了几种medium，要么长的慢，要么集团。谢谢
| m*********D
发帖数: 1727 | 4 We use DMEM plus 10% FBS.
Yes, the cells clump. We pass the cell suspension through 20G needle twice
when we split, especially when we need to count cell number.
However, based on my own experience, after 20 passes, the cells tend to loss
some characters of liver cells. | g*********5
发帖数: 2533 | 5 neither of them work well
mem +10%FBS
DMEM+10%FBS
F12+10%FBS
F12/DMEM+10%FBS
【在 s*****g 的大作中提到】
: 是mem还是dmem?
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