s********9
发帖数: 132 | 1 When people say there are hundreds E3 in the proteome, do they know each and
every one in these hundreds has proven activity
towards some sort of substrate? Otherwise where does this number (~ one
thousand or some say ~600) come from?
Also,
For SCF, people say there are hundreds of them, do they know experimentally
the S, C, and F of each SCF? otherwise where does
this number (few hundres SCF) come from? (Is it the theoretical combinations
of all S,C,F?)
The same question of CRL. Do they know the C and R and other components of
these complexes? | s*********y
发帖数: 292 | 2 纯属理论预测。根据有多少个F-box protein之类的算出来的
and
experimentally
combinations
【在 s********9 的大作中提到】
: When people say there are hundreds E3 in the proteome, do they know each and
: every one in these hundreds has proven activity
: towards some sort of substrate? Otherwise where does this number (~ one
: thousand or some say ~600) come from?
: Also,
: For SCF, people say there are hundreds of them, do they know experimentally
: the S, C, and F of each SCF? otherwise where does
: this number (few hundres SCF) come from? (Is it the theoretical combinations
: of all S,C,F?)
: The same question of CRL. Do they know the C and R and other components of
| s********9
发帖数: 132 | 3 thanks, any chance you know any reference?
【在 s*********y 的大作中提到】
: 纯属理论预测。根据有多少个F-box protein之类的算出来的
:
: and
: experimentally
: combinations
| S*******r
发帖数: 530 | 4 1. 有RING domain的被假定为E3，通过genome wide search
2. 能autoubiquitylation的被认为是E3
3. 很多E3还没有发现substrate |
|
