r**********e 发帖数: 587 | 1 I don't know anyone here has experience with plasmid transfection fir SH-
SY5Y?
Lipofectamine 3000 doesn't work at all.
Neuronal line is always challenging for transfection.
Any recommendations of good reagent to achieve relatively high transfection
efficiency?
Thx! |
d******n 发帖数: 3836 | |
j*****9 发帖数: 716 | 3 I have tried about 10 lipid-based transfection reagents in SH-SY5Y and
neurons. None of them gave me a decent efficiency. NeuroPORTER (Genlantis or
Sigma-Aldrich) was the best (~30% in SH-SY5Y, 18% in Neurons using FACS).
Good luck
transfection
【在 r**********e 的大作中提到】![](/moin_static193/solenoid/img/up.png) : I don't know anyone here has experience with plasmid transfection fir SH- : SY5Y? : Lipofectamine 3000 doesn't work at all. : Neuronal line is always challenging for transfection. : Any recommendations of good reagent to achieve relatively high transfection : efficiency? : Thx!
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U*****e 发帖数: 40 | 4 我们公司自己开发了一种转染试剂,同现在市售的转染试剂都不同,比较广谱,相对各
种核酸及细胞都可以用。而且可以用荧光实时检测转染试剂同质粒符合物的生成及转染
细胞的过程。产品还没用放入公司网页。如果你想试试,我们可以提供试用样品。
Bioandbeyond.com |
c**i 发帖数: 265 | 5 我们lab用FuGene HD,效率一般。现在准备做lenti |
c*******s 发帖数: 29 | 6 Xfect from Clontech is relatively the best in our hand.
transfection
【在 r**********e 的大作中提到】![](/moin_static193/solenoid/img/up.png) : I don't know anyone here has experience with plasmid transfection fir SH- : SY5Y? : Lipofectamine 3000 doesn't work at all. : Neuronal line is always challenging for transfection. : Any recommendations of good reagent to achieve relatively high transfection : efficiency? : Thx!
|
s*****g 发帖数: 7857 | 7 lonza电转
即使用其他公司试剂(Mirus)也行
我曾经也面对过难转染的细胞 |
U*****e 发帖数: 40 | 8 Updated link: visible transfection
http://bioandbeyond.com/p/whats-new |
r**********e 发帖数: 587 | 9 Hi Thanks
For NeuroPORTER
Genlantis or Sigma-Aldrich, which one is better? And why the same product
but two companies? And I don't see Genlantis is being merged by Sigma
or
【在 j*****9 的大作中提到】![](/moin_static193/solenoid/img/up.png) : I have tried about 10 lipid-based transfection reagents in SH-SY5Y and : neurons. None of them gave me a decent efficiency. NeuroPORTER (Genlantis or : Sigma-Aldrich) was the best (~30% in SH-SY5Y, 18% in Neurons using FACS). : Good luck : : transfection
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