r***a 发帖数: 7 | 1 Hi,
I treated groups of transgenic mice with a few chemicals for inhibiting
cancer but noticed one chemical may reduce the activity of insulin. A
direct consequence should be higher blood glucose level/increased insulin.
But the experiment has been completed and there is not much chance to
convince my boss to repeat an experiment for measure blood glucose level. I
am not in the metabolism field but found that most works in the
publications measure the glucose after overnight fasting or at least using
fresh blood drops. Can I use the frozen serum (collected for measure
cytokines) to measure blood glucose levels?
Thanks! |
r******g 发帖数: 600 | 2 should be ok in theory. But I have never tried frozen serum. Just buy a
glucose meter from pharmacy. It works fine in our hands for mice serum. |
D*a 发帖数: 6830 | 3 测糖尿病人的血糖仪,尾巴拿针头扎一下,挤血,捏到血糖仪纸条上。
一只老鼠可以给药前后测,还可以隔几分钟、几小时、天天测,statistic power大大
好于你去冰箱里面捞血清。
找个你们楼做糖尿病的实验室去聊聊。
血清也不是不行,但是糖尿病血糖仪有校准,你拿它测血清和拿它测全血不能直接比。 |
D*a 发帖数: 6830 | 4 不fasting的问题是老鼠吃饭都不定点,浮动明显比fasting的大,不过也还可以,你药
明显的话肯定没问题,如果你要fasting,记得换新笼子,因为老鼠吃粑粑 |
l***y 发帖数: 638 | 5 找个做diabetes的问问,测这个都是routine的
I
【在 r***a 的大作中提到】![](/moin_static193/solenoid/img/up.png) : Hi, : I treated groups of transgenic mice with a few chemicals for inhibiting : cancer but noticed one chemical may reduce the activity of insulin. A : direct consequence should be higher blood glucose level/increased insulin. : But the experiment has been completed and there is not much chance to : convince my boss to repeat an experiment for measure blood glucose level. I : am not in the metabolism field but found that most works in the : publications measure the glucose after overnight fasting or at least using : fresh blood drops. Can I use the frozen serum (collected for measure : cytokines) to measure blood glucose levels?
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h*******o 发帖数: 4884 | 6 serum glucose测试没问题, frozen sample也可以用
但是如果你当时sac 老鼠的时候没有fast, 那么需要很大的N per group 才能检测到
overall的区别
老鼠隔三差五就会去啃一口mouse chow, 所以blood glucose 变化会比较大 除非你的
药像streptozotocin这种强效。 |
W****7 发帖数: 426 | 7 Go ahead give a try. Should be fine. If you do have any problem, we have a
machine for measuring glucose level in serum. |
C*********u 发帖数: 811 | 8 我试过用serum测过glucose,大概比用新鲜的血测高一倍左右。
应该是因为血里面大概一半的体积是血细胞。
I
【在 r***a 的大作中提到】![](/moin_static193/solenoid/img/up.png) : Hi, : I treated groups of transgenic mice with a few chemicals for inhibiting : cancer but noticed one chemical may reduce the activity of insulin. A : direct consequence should be higher blood glucose level/increased insulin. : But the experiment has been completed and there is not much chance to : convince my boss to repeat an experiment for measure blood glucose level. I : am not in the metabolism field but found that most works in the : publications measure the glucose after overnight fasting or at least using : fresh blood drops. Can I use the frozen serum (collected for measure : cytokines) to measure blood glucose levels?
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z*t 发帖数: 863 | 9 我用frozen serum做过metablomics没有fasting的情况下没有特别大的variation,抽
血的时间也不定时
不过我是做的leukemia mice, 跟control进行比较,如果要测很准的血糖还是要小心
I
【在 r***a 的大作中提到】![](/moin_static193/solenoid/img/up.png) : Hi, : I treated groups of transgenic mice with a few chemicals for inhibiting : cancer but noticed one chemical may reduce the activity of insulin. A : direct consequence should be higher blood glucose level/increased insulin. : But the experiment has been completed and there is not much chance to : convince my boss to repeat an experiment for measure blood glucose level. I : am not in the metabolism field but found that most works in the : publications measure the glucose after overnight fasting or at least using : fresh blood drops. Can I use the frozen serum (collected for measure : cytokines) to measure blood glucose levels?
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