c********6
发帖数: 693 | 1 现在杨辉发表的
Nature Methods:
A rationally engineered cytosine base editor retains high on-target activity
while reducing both DNA and RNA off-target effects
Erwei Zuo 1,2,10 ✉, Yidi Sun 3,4,10, Tanglong Yuan 1,10,
Bingbing He 2,10, Changyang Zhou 2,10, Wenqin Ying 2, Jing Liu 1, Wu
Wei 4,5, Rong Zeng3,6, Yixue Li 4,6,7,8 ✉ and Hui Yang 2,9 &#
9993;
Cytosine base editors (CBEs) offer a powerful tool for correcting point
mutations, yet their DNA and RNA off-target activities have caused concerns
in biomedical applications. We describe screens of 23 rationally engineered
CBE variants, which reveal mutation residues in the predicted DNA-binding
site can dra- matically decrease the Cas9-independent off-target effects.
Furthermore, we obtained a CBE variant—YE1-BE3-FNLS— that retains high on-
target editing efficiency while causing extremely low off-target edits and
bystander edits.
去年杨辉发表的
Sicence:
Cytosine base editor generates substantial off-target single-nucleotide
variants in mouse embryos
Erwei Zuo1,2,*, Yidi Sun3,*, Wu Wei4,5,6,*, Tanglong Yuan2,*, Wenqin Ying1,
Hao Sun7, Liyun Yuan4, Lars M. Steinmetz5,8,9,†, Yixue Li4,10,11,&#
8224;, Hui Yang1,†
Abstract
Genome editing holds promise for correcting pathogenic mutations. However,
it is difficult to determine off-target effects of editing due to single-
nucleotide polymorphism in individuals. Here we developed a method named
GOTI (genome-wide off-target analysis by two-cell embryo injection) to
detect off-target mutations by editing one blastomere of two-cell mouse
embryos using either CRISPR-Cas9 or base editors. Comparison of the whole-
genome sequences of progeny cells of edited and nonedited blastomeres at
embryonic day 14.5 showed that off-target single-nucleotide variants (SNVs)
were rare in embryos edited by CRISPR-Cas9 or adenine base editor, with a
frequency close to the spontaneous mutation rate. By contrast, cytosine base
editing induced SNVs at more than 20-fold higher frequencies, requiring a
solution to address its fidelity.
各位怎么看? 杨大师发功往往能够化腐朽为神奇! | g***s
发帖数: 30 | 2 不是内行，看了一些文章的简介，说实话，没看出有什么问题,science文的设计也很精巧
activity
Wu
concerns
【在 c********6 的大作中提到】
: 现在杨辉发表的
: Nature Methods:
: A rationally engineered cytosine base editor retains high on-target activity
: while reducing both DNA and RNA off-target effects
: Erwei Zuo 1,2,10 ✉, Yidi Sun 3,4,10, Tanglong Yuan 1,10,
: Bingbing He 2,10, Changyang Zhou 2,10, Wenqin Ying 2, Jing Liu 1, Wu
: Wei 4,5, Rong Zeng3,6, Yixue Li 4,6,7,8 ✉ and Hui Yang 2,9 &#
: 9993;
: Cytosine base editors (CBEs) offer a powerful tool for correcting point
: mutations, yet their DNA and RNA off-target activities have caused concerns
| f*******e
发帖数: 354 | 3 给杂志社editor写信或者pubpeer公开质疑吧，在这儿阴阳怪气的显不出本事。 | c********6
发帖数: 693 | 4 自己有本事造文章就不要怕别人质疑!!!
【在 f*******e 的大作中提到】
: 给杂志社editor写信或者pubpeer公开质疑吧，在这儿阴阳怪气的显不出本事。
| c********6
发帖数: 693 | |
|
