Biology版 - 翻手为云覆手为雨, Johns Hopkins, Ted Dawson et al. |
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b******e
发帖数: 61 | 1 生物学界的高水平造假，看大家如何编故事，讲故事好听的吗？
一个广为接受的事实：LAG3 （a checkpoint molecule), 不管是否在别的细胞上表达
与否, 应该至少在小胶质细胞，免疫细胞上表达，这个Johns Hopkins, Ted Dawson
and Xiaobo Mao 2016年‘有大发现’，发了一篇 Science，“Pathological α-
synuclein transmission initiated by binding lymphocyte-activation gene 3”
【Science (2016) pubmed: 27708076，doi: 10.1126/science.aah3374】，声称 ‘
LAG3 was primarily expressed in neurons because LAG3 was not detectable in
astrocytes or microglia’. 审稿人水平也太不敢恭维了。
同一个组同一位一作，估计是看到2016年的他们自己的发现难以自圆谎（？），Xiaobo
Mao 在一篇新的预印版 they show the expression of LAG3 in microglia. (Aplp1
and the Aplp1-Lag3 Complex facilitates transmission of pathologic α-
synuclein, https://
www.biorxiv.org/content/10.1101/2021.05.01.442157v1)，翻手为云覆手为雨啊。
看来发大paper 拿到 grant 是王道，哪管事情真伪啊。
现在是一个瑞士的实验组发现 LAG3 不但不在神经元上表达，而且发现 LAG3根本就没
有这个Johns Hopkins, Ted Dawson and Xiaobo Mao 发现的调节synucleinopathies的
作用， LAG3 is
not expressed in human and murine neurons and does not modulate α-
synucleinopathies" https://www.biorxiv.org/content/10.1101/2021.04.25.
441302v1 )
-----------------------------------------------------------------------------
https://pubpeer.com/publications/6DE486C75FB8FC5C057D72B27C926E
The same group and the same first author, they claimed in their 2016 Science
paper, that ‘LAG3 was primarily expressed in neurons because LAG3 was not
detectable in astrocytes or microglia’. (Science. 2016 Sep 30; 353(6307):
aah3374),
So many good labs had CLEARLY shown and approved that LAG3 is thought to be
largely in microglia. If the LAG3 antibodies they used were specific, LAG3
expression in microglia should be detected in their 2016 Science paper, why
they say "LAG3 was expressed in neurons but not in microglia?
Now, Mao et al NEW preprint, they show the expression of LAG3 in microglia.
(Aplp1 and the Aplp1-Lag3 Complex facilitates transmission of pathologic α-
synuclein, https://www.biorxiv.org/content/10.1101/2021.05.01.442157v1 )
And interestingly, in this preprint (Aplp1 and the Aplp1-Lag3 Complex
facilitates transmission of pathologic α-synuclein, https://www.biorxiv.org
/content/10.1101/2021.05.01.442157v1 ), using LAG3-YFP transgenic mice to
verify LAG3 protein expression in (mouse) neuron look good, but why the GFP
antibody was used to stain YFP? Artifacts? The anti-GFP staining pattern was
not convincing, if you look their nuclei counterstaining, it seems very
like auto-fluorescence. Can this truly approve the neuronal expression of
LAG3, why not do solid and convincing studies like another preprint "LAG3 is
not expressed in human and murine neurons and does not modulate α-
synucleinopathies" https://www.biorxiv.org/content/10.1101/2021.04.25.
441302v1 )
"The immunoreactivity of anti-GFP, which recognizes YFP co-localized with
MAP2 immunostaining in the spinal cord, olfactory bulb, striatum, substantia
nigra pars compacta, hippocampus, cortex, cerebellum and brainstem (fig.
S3A). The lack of immunoreactivity of anti-GFP in WT mice indicates that the
anti-GFP immunoreactivity is specific in Lag3L/L-YFP mice (fig. S3B). In
addition, anti-GFP colocalizes with IBA1 immunostaining, but not GFAP
immunostaining (fig. S3B)" | r***i
发帖数: 1 | 2 编造这个意义何在？
生物千老随便泡个脚就能证明蛋白有没有吧
作假做的水平高点不好吗？
盹盹盹
Xiaobo
Aplp1
---
Science
be
why
α-
org
GFP
was
is
substantia
the
【在 b******e 的大作中提到】
: 生物学界的高水平造假，看大家如何编故事，讲故事好听的吗？
: 一个广为接受的事实：LAG3 （a checkpoint molecule), 不管是否在别的细胞上表达
: 与否, 应该至少在小胶质细胞，免疫细胞上表达，这个Johns Hopkins, Ted Dawson
: and Xiaobo Mao 2016年‘有大发现’，发了一篇 Science，“Pathological α-
: synuclein transmission initiated by binding lymphocyte-activation gene 3”
: 【Science (2016) pubmed: 27708076，doi: 10.1126/science.aah3374】，声称 ‘
: LAG3 was primarily expressed in neurons because LAG3 was not detectable in
: astrocytes or microglia’. 审稿人水平也太不敢恭维了。
: 同一个组同一位一作，估计是看到2016年的他们自己的发现难以自圆谎（？），Xiaobo
: Mao 在一篇新的预印版 they show the expression of LAG3 in microglia. (Aplp1
| b******e
发帖数: 61 | 3 为了名和利吧： fame and money，学术界不就是教授的title，grant。
虽然这个发现赶不上韩春雨的NgAgo, 但是也是大发现啊，对于一个以 synuclein 为病
理特征的帕金森氏疾病是一个巨大突破，想想只要阻断这个LAG3，不就搞定了吗？
【在 r***i 的大作中提到】
: 编造这个意义何在？
: 生物千老随便泡个脚就能证明蛋白有没有吧
: 作假做的水平高点不好吗？
: 盹盹盹
:
: Xiaobo
: Aplp1
: ---
: Science
: be
| E******k
发帖数: 389 | | b******e
发帖数: 61 | 5 这个算是编造故事吧
【在 E******k 的大作中提到】
: 没有被造假举报吗
| b******e
发帖数: 61 | 6 生物学界的高水平造假，看大家如何编故事，讲故事好听的吗？
一个广为接受的事实：LAG3 （a checkpoint molecule), 不管是否在别的细胞上表达
与否, 应该至少在小胶质细胞，免疫细胞上表达，这个Johns Hopkins, Ted Dawson
and Xiaobo Mao 2016年‘有大发现’，发了一篇 Science，“Pathological α-
synuclein transmission initiated by binding lymphocyte-activation gene 3”
【Science (2016) pubmed: 27708076，doi: 10.1126/science.aah3374】，声称 ‘
LAG3 was primarily expressed in neurons because LAG3 was not detectable in
astrocytes or microglia’. 审稿人水平也太不敢恭维了。
同一个组同一位一作，估计是看到2016年的他们自己的发现难以自圆谎（？），Xiaobo
Mao 在一篇新的预印版 they show the expression of LAG3 in microglia. (Aplp1
and the Aplp1-Lag3 Complex facilitates transmission of pathologic α-
synuclein, https://
www.biorxiv.org/content/10.1101/2021.05.01.442157v1)，翻手为云覆手为雨啊。
看来发大paper 拿到 grant 是王道，哪管事情真伪啊。
现在是一个瑞士的实验组发现 LAG3 不但不在神经元上表达，而且发现 LAG3根本就没
有这个Johns Hopkins, Ted Dawson and Xiaobo Mao 发现的调节synucleinopathies的
作用， LAG3 is
not expressed in human and murine neurons and does not modulate α-
synucleinopathies" https://www.biorxiv.org/content/10.1101/2021.04.25.
441302v1 )
-----------------------------------------------------------------------------
https://pubpeer.com/publications/6DE486C75FB8FC5C057D72B27C926E
The same group and the same first author, they claimed in their 2016 Science
paper, that ‘LAG3 was primarily expressed in neurons because LAG3 was not
detectable in astrocytes or microglia’. (Science. 2016 Sep 30; 353(6307):
aah3374),
So many good labs had CLEARLY shown and approved that LAG3 is thought to be
largely in microglia. If the LAG3 antibodies they used were specific, LAG3
expression in microglia should be detected in their 2016 Science paper, why
they say "LAG3 was expressed in neurons but not in microglia?
Now, Mao et al NEW preprint, they show the expression of LAG3 in microglia.
(Aplp1 and the Aplp1-Lag3 Complex facilitates transmission of pathologic α-
synuclein, https://www.biorxiv.org/content/10.1101/2021.05.01.442157v1 )
And interestingly, in this preprint (Aplp1 and the Aplp1-Lag3 Complex
facilitates transmission of pathologic α-synuclein, https://www.biorxiv.org
/content/10.1101/2021.05.01.442157v1 ), using LAG3-YFP transgenic mice to
verify LAG3 protein expression in (mouse) neuron look good, but why the GFP
antibody was used to stain YFP? Artifacts? The anti-GFP staining pattern was
not convincing, if you look their nuclei counterstaining, it seems very
like auto-fluorescence. Can this truly approve the neuronal expression of
LAG3, why not do solid and convincing studies like another preprint "LAG3 is
not expressed in human and murine neurons and does not modulate α-
synucleinopathies" https://www.biorxiv.org/content/10.1101/2021.04.25.
441302v1 )
"The immunoreactivity of anti-GFP, which recognizes YFP co-localized with
MAP2 immunostaining in the spinal cord, olfactory bulb, striatum, substantia
nigra pars compacta, hippocampus, cortex, cerebellum and brainstem (fig.
S3A). The lack of immunoreactivity of anti-GFP in WT mice indicates that the
anti-GFP immunoreactivity is specific in Lag3L/L-YFP mice (fig. S3B). In
addition, anti-GFP colocalizes with IBA1 immunostaining, but not GFAP
immunostaining (fig. S3B)" | r***i
发帖数: 1 | 7 编造这个意义何在？
生物千老随便泡个脚就能证明蛋白有没有吧
作假做的水平高点不好吗？
盹盹盹
Xiaobo
Aplp1
---
Science
be
why
α-
org
GFP
was
is
substantia
the
【在 b******e 的大作中提到】
: 生物学界的高水平造假，看大家如何编故事，讲故事好听的吗？
: 一个广为接受的事实：LAG3 （a checkpoint molecule), 不管是否在别的细胞上表达
: 与否, 应该至少在小胶质细胞，免疫细胞上表达，这个Johns Hopkins, Ted Dawson
: and Xiaobo Mao 2016年‘有大发现’，发了一篇 Science，“Pathological α-
: synuclein transmission initiated by binding lymphocyte-activation gene 3”
: 【Science (2016) pubmed: 27708076，doi: 10.1126/science.aah3374】，声称 ‘
: LAG3 was primarily expressed in neurons because LAG3 was not detectable in
: astrocytes or microglia’. 审稿人水平也太不敢恭维了。
: 同一个组同一位一作，估计是看到2016年的他们自己的发现难以自圆谎（？），Xiaobo
: Mao 在一篇新的预印版 they show the expression of LAG3 in microglia. (Aplp1
| b******e
发帖数: 61 | 8 为了名和利吧： fame and money，学术界不就是教授的title，grant。
虽然这个发现赶不上韩春雨的NgAgo, 但是也是大发现啊，对于一个以 synuclein 为病
理特征的帕金森氏疾病是一个巨大突破，想想只要阻断这个LAG3，不就搞定了吗？
【在 r***i 的大作中提到】
: 编造这个意义何在？
: 生物千老随便泡个脚就能证明蛋白有没有吧
: 作假做的水平高点不好吗？
: 盹盹盹
:
: Xiaobo
: Aplp1
: ---
: Science
: be
| E******k
发帖数: 389 | | b******e
发帖数: 61 | 10 这个算是编造故事吧
【在 E******k 的大作中提到】
: 没有被造假举报吗
| b******e
发帖数: 61 | 11 这个实验室的另外一篇Science又有问题，这次是Tae-In Kam1,2,3*, Xiaobo Mao1,2,3
*...， 这是老板的问题？还是一作编造美丽的故事？这个Xiaobo Mao的两篇 Science
， 都是美丽的谎言啊。
The primary purpose of this study was to replicate the key end-points
related to the efficacy of ABT-888 in the amelioration of α-synuclein
fibril-induced phenotypes in vitro and in vivo as principally reported by
Kam et al.20 While our models met the expected variabilities and end-point
lesion sizes, α-synuclein inclusion loads were comparable to those
previously reported for α-synuclein fibril-seeding-based models,5,7,30–32
we were unable to resolve beneficial effects related to ABT-888 treatments.
'Evaluation of ABT-888 in the amelioration of α-synuclein fibril-induced
neurodegeneration'. Lyndsay Hastings, Arpine Sokratian, Daniel J. Apicco,
Christina M. Stanhope, Lindsey Smith, Warren D. Hirst, Andrew B. West, Kaela
Kelly. Brain Communications, Volume 4, Issue 2, 2022, fcac042 | f*****e
发帖数: 34 | 12 Xiaobo Mao已经是Associate Professor of Neurology，洗洗睡吧。 | b******e
发帖数: 61 | 13 这还还真是的和，妈蛋，rocket， 您认识他吗？
【在 f*****e 的大作中提到】
: Xiaobo Mao已经是Associate Professor of Neurology，洗洗睡吧。
| p*******e
发帖数: 1 | 14 流弊。北京工商大学高分子系的。
: 这还还真是的和，妈蛋，rocket， 您认识他吗？
【在 b******e 的大作中提到】
: 这还还真是的和，妈蛋，rocket， 您认识他吗？
| b******e
发帖数: 61 | 15 这个实验室的另外一篇Science又有问题，这次是Tae-In Kam1,2,3*, Xiaobo Mao1,2,3
*...， 这是老板的问题？还是一作编造美丽的故事？这个Xiaobo Mao的两篇 Science
， 都是美丽的谎言啊。
The primary purpose of this study was to replicate the key end-points
related to the efficacy of ABT-888 in the amelioration of α-synuclein
fibril-induced phenotypes in vitro and in vivo as principally reported by
Kam et al.20 While our models met the expected variabilities and end-point
lesion sizes, α-synuclein inclusion loads were comparable to those
previously reported for α-synuclein fibril-seeding-based models,5,7,30–32
we were unable to resolve beneficial effects related to ABT-888 treatments.
'Evaluation of ABT-888 in the amelioration of α-synuclein fibril-induced
neurodegeneration'. Lyndsay Hastings, Arpine Sokratian, Daniel J. Apicco,
Christina M. Stanhope, Lindsey Smith, Warren D. Hirst, Andrew B. West, Kaela
Kelly. Brain Communications, Volume 4, Issue 2, 2022, fcac042 | f*****e
发帖数: 34 | 16 Xiaobo Mao已经是Associate Professor of Neurology，洗洗睡吧。 | b******e
发帖数: 61 | 17 这还还真是的和，妈蛋，rocket， 您认识他吗？
【在 f*****e 的大作中提到】
: Xiaobo Mao已经是Associate Professor of Neurology，洗洗睡吧。
| p*******e
发帖数: 1 | 18 流弊。北京工商大学高分子系的。
: 这还还真是的和，妈蛋，rocket， 您认识他吗？
【在 b******e 的大作中提到】
: 这还还真是的和，妈蛋，rocket， 您认识他吗？
| b******e
发帖数: 61 | 19 北京工商大学高分子系, 是个什么level的？
【在 p*******e 的大作中提到】
: 流弊。北京工商大学高分子系的。
:
:
: 这还还真是的和，妈蛋，rocket， 您认识他吗？
:
| L***C
发帖数: 1 | 20 严格起来的话，nature和science上很多文章要撤稿。 你要是再看那些data perfect的
circRNA和lncRNA文章，有几篇能完全重复呢？ 太认真的话，就输在起跑线上了！ | b******e
发帖数: 61 | 21 这比直接在western blot 上直接画条带更优解，骗NIH的grant 一愣一愣的。
【在 E******k 的大作中提到】
: 没有被造假举报吗
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